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Longya lilium detoxification method

A technology of disinfection and sterilization, culture medium formulation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as quality and yield decline, hindering healthy development, and seed bulb degradation.

Pending Publication Date: 2021-03-12
COTTON SCI RES INST OF HUNAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the development of the industry, there is always a core scientific problem that hinders its healthy development: that is, the accumulation of viruses and bacteria caused by the production method of saving seeds for hundreds of years, the degradation of bulbs, and the decline in quality and yield

Method used

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  • Longya lilium detoxification method

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Effect test

Embodiment

[0020] The detoxification method of dragon tooth lily includes the following steps:

[0021] Disinfection and sterilization of the tissue culture room: dilute to 1000 times with 84 disinfectant or spray and wash the ground of the tissue culture room with 75% ethanol, keep the room clean, fumigate with potassium permanganate and hypochlorous acid every 30 days, 15 days before inoculation in the inoculation room Irradiate with ultraviolet light for 30 minutes, and irradiate the ultra-clean workbench with ultraviolet light for 30 minutes before inoculation, then turn off the ultraviolet light and ventilate for more than 20 minutes, gently clean the surrounding area of ​​the ultra-clean workbench with a wet mop to reduce dust, and then wipe the operating area of ​​the worktop with 75% ethanol ;

[0022] S2. Culture medium preparation and treatment: a. Culture medium preparation, the basic medium is MS medium, 80 g / L of added sugar is added for the primary culture, and 80 g / L of ad...

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Abstract

The invention discloses a longya lilium detoxification method. The longya lilium detoxification method comprises the following steps of S1, disinfecting and sterilizing in a tissue culture room, specifically, using 84 disinfectant diluted to 1000 times or 75% ethanol for spraying and washing the ground of the tissue culture room, keeping the room clean, performing mixed fumigation with potassium permanganate and hypochlorous acid every 30 days, irradiating with an ultraviolet lamp for 30 minutes 15 days before inoculation in an inoculation room, irradiating with an ultraviolet lamp for 30 minutes before inoculation in an ultra-clean workbench, then turning off the ultraviolet lamp, ventilating for more than 20 minutes, lightly cleaning the periphery of the ultra-clean workbench by using awet mop to reduce dust, and wiping an operation area of the workbench surface by using 75% ethanol; and S2, preparation and treatment of a culture medium, specifically, a, preparation of the culture medium, specifically, taking an MS culture medium as a basic culture medium, adding 80g / L of sugar to primary culture, and adding 80g / L of sugar to other culture medium formulas except special instructions. Healthy and detoxified lily bulbs obtained by the longya lilium detoxification method are produced on a large scale in a commercialized manner, the production cost is reduced, meanwhile, the problems that the bulbs are poisonous and deteriorated, and the quality is reduced can be effectively solved, and lily yield increase and farmer income increase are promoted.

Description

technical field [0001] The invention relates to the technical field of cultivating Lily Longya, in particular to a detoxification method for Lily Longya. Background technique [0002] Lily of dragon teeth, spherical bulb, 2-2.4 cm in transverse diameter, scales lanceolate, white, yellowish white or slightly pink when exposed to sunlight. High starch content, rich nutrition, light taste but not bitter, the stems above the ground are about 100 cm high, and the leaves are scattered and oblanceolate. The flowers are milky white and fragrant, and can produce seeds. Distributed in my country's Hunan, Hubei, Jiangxi, Zhejiang, Henan, Hebei, Shaanxi and other provinces at an altitude of 200-390 meters. Longya lily products are tributes of all dynasties, and it is an excellent variety of white lily. [0003] There is a huge demand gap for lily products in domestic and foreign markets, and the industry has great potential for development. However, there has always been a core scie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008A01H4/00
Inventor 马杰傅淋王永波贺璐李庠肖才升曾潜
Owner COTTON SCI RES INST OF HUNAN