Longya lilium detoxification method
A technology of disinfection and sterilization, culture medium formulation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as quality and yield decline, hindering healthy development, and seed bulb degradation.
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[0020] The detoxification method of dragon tooth lily includes the following steps:
[0021] Disinfection and sterilization of the tissue culture room: dilute to 1000 times with 84 disinfectant or spray and wash the ground of the tissue culture room with 75% ethanol, keep the room clean, fumigate with potassium permanganate and hypochlorous acid every 30 days, 15 days before inoculation in the inoculation room Irradiate with ultraviolet light for 30 minutes, and irradiate the ultra-clean workbench with ultraviolet light for 30 minutes before inoculation, then turn off the ultraviolet light and ventilate for more than 20 minutes, gently clean the surrounding area of the ultra-clean workbench with a wet mop to reduce dust, and then wipe the operating area of the worktop with 75% ethanol ;
[0022] S2. Culture medium preparation and treatment: a. Culture medium preparation, the basic medium is MS medium, 80 g / L of added sugar is added for the primary culture, and 80 g / L of ad...
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