A kind of compound microbial bacterial agent for potato black mole
A compound microbial inoculant, a technology for potato black nevus, which is applied in the field of agricultural microorganisms and can solve the problems of unstable control effect and the like
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Embodiment 1
[0039] The preparation method of the composite microbial inoculant of preventing and treating potato black mole, the steps are as follows:
[0040] (1) Inoculate Bacillus atrophaeus CICC 20238 in LB liquid medium, and culture it in a shaker flask for 36 hours at 37°C to obtain liquid seeds, inoculate the obtained liquid seeds in liquid fermentation medium A, and adjust the pH value to 7.0, the aeration ratio is 1:1, the rotating speed is 170 rev / min, cultivated at 37°C for 36h to obtain a fermented liquid, and the fermented liquid is centrifuged and dried to obtain an effective viable count of 5 billion / g of solid microbial agent A;
[0041] (2) Bacillus polymyxa ACCC 10122 was inoculated in LB liquid medium, and cultured in shake flasks for 40 hours at 35°C to obtain liquid seeds, which were inoculated into liquid fermentation medium B, and regulated The pH value is 7.0, the ventilation ratio is 1:0.75, and the rotation speed is 150 rpm. Cultivate at 35°C for 24 hours to ob...
Embodiment 2
[0052] Adopt the method as described in embodiment 1, difference is, by weight, 7 parts of solid microbial agent A, 20 parts of solid microbial agent B, 30 parts of solid microbial agent C are mixed in step (4).
[0053] In the prepared composite microbial agent for preventing and treating potato black mole, each gram of the composite microbial agent contains: Bacillus atrophaeus CICC 20238 effective viable count 600 million / g, Bacillus polymyxa ACCC 10122 effective viable count 17 100 million / g, Trichoderma harzianum ACCC 33109 effective viable count is 1.5 billion / g, total effective count is 3.8 billion / g.
Embodiment 3
[0055] Adopt the method as described in embodiment 1, difference is, in step (4), 10 parts of solid microbial agent A, 20 parts of solid microbial agent B, 30 parts of solid microbial agent C are mixed by weight.
[0056] In the prepared composite microbial agent for preventing and treating potato black mole, each gram of the composite microbial agent contains: Bacillus atrophaeus CICC 20238 effective viable count 800 million / g, Bacillus polymyxa ACCC 10122 effective viable count 16 100 million / g, Trichoderma harzianum ACCC 33109 effective viable count 1.5 billion / g, total effective count 3.9 billion / g.
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