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Trichoderma koningiopsis for antagonizing various pathogenic fungi of potatoes and application of trichoderma koningiopsis

A technology of Trichoderma, potato, applied in the field of microorganisms

Active Publication Date: 2021-10-22
黑龙江省农业科学院马铃薯研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Different diseases cause different degrees of damage to potatoes, and there are many biocontrol bacterial species that are found to be antagonistic to potato late blight, and relatively few antagonistic bacterial species to other fungal diseases of potato. Late blight, and other biocontrol strains that can prevent and control other potato fungal diseases are of great significance to solve the problems existing in potato planting

Method used

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  • Trichoderma koningiopsis for antagonizing various pathogenic fungi of potatoes and application of trichoderma koningiopsis
  • Trichoderma koningiopsis for antagonizing various pathogenic fungi of potatoes and application of trichoderma koningiopsis
  • Trichoderma koningiopsis for antagonizing various pathogenic fungi of potatoes and application of trichoderma koningiopsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Trichoderma obtains isolation, purification and identification

[0032] 1. Take soil samples evenly from the cornfield in Hulan District, Harbin City, Heilongjiang Province, weigh 10g of the collected soil samples, add 90ml of sterile water, and shake at room temperature for 30min at 150rpm. Afterwards, draw the supernatant and adopt 10-fold gradient dilution method to carry out the separation of biocontrol Trichoderma, each selective culture medium (Red Bengal medium: peptone 5g, glucose 10g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, agar 20g, 1 / 3000 Bengal red solution 100mL, distilled water 1000mL) uniformly spread 100μl soil dilution on the plate. Then, at 10 -3 Single colonies were picked on the dilution plate and purified on PDA medium (200 g of potatoes, 20 g of glucose, 15 g of agar, distilled water to 1000 ml) for classification and identification. The strains were morphologically determined according to the Fungal Identification...

Embodiment 2

[0050] The antibacterial effect of embodiment 2 Trichoderma TC24 of the present invention

[0051] 1. Materials and methods

[0052] Pathogenic fungi tested: 2 strains of Rhizoctonia solani whose fusion groups are AG3 and AG5; 1 strain of Fusarium elderberry; 1 strain of Alternaria solani.

[0053] Method: The inhibitory effect of Trichoderma TC24 on 4 kinds of tested pathogenic fungi was identified by plate confrontation culture method.

[0054] The bacteria cakes (about 6mm in diameter) of Trichoderma TC24 and pathogenic fungi were taken respectively by a puncher for confrontation culture. Treatment group: On a PDA plate (80 mm in diameter), one Trichoderma TC24 cake and one pathogenic fungus cake were inoculated face to face, and the distance between them was 40 mm. Control group: Trichoderma TC24 and pathogenic fungi were inoculated on independent PDA plates, respectively. Except for Alternaria solani which was cultured at 23℃, the confrontation culture of the other thr...

Embodiment 3

[0062] Embodiment 3 Trichoderma TC24 microbial inoculum suppresses pathogenic fungus onset

[0063] 1. Preparation of Trichoderma TC24 microbial inoculum

[0064] The above isolated and purified Trichoderma TC24 was inoculated into PDA liquid medium (200 g of potatoes, 20 g of glucose, and distilled water to a constant volume of 1000 ml), 25° C., 150 rpm shaking culture for 1 day, and the hyphae were filtered out with sterile filter paper to prepare Trichoderma spore suspension Liquid, promptly obtains Trichoderma TC24 microbial inoculum.

[0065] 2. Antibacterial effect of Trichoderma TC24 microbial agent

[0066] 2.1 Materials and methods

[0067] Cut the potato tubers into 20mm×20mm, 10mm thick uniform potato pieces with a slicer, soak in deionized water to wash off the starch on the surface, sterilize the surface with 1% NaClO for 5min, and rinse with sterile deionized water for 3 times. Soak the potato pieces in Trichoderma spore suspension for 30 minutes, take them ou...

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Abstract

The invention discloses trichoderma koningiopsis for antagonizing various pathogenic fungi of potatoes and application of the trichoderma koningiopsis, and belongs to the technical field of microorganisms. The invention discloses trichoderma koningiopsis, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 23, 2021, the preservation number is CGMCC No.23070, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The invention further discloses application of the trichoderma koningiopsis and a microbial agent containing the trichoderma koningiopsisto in the prevention and treatment of plant diseases and application to bacteriostasis. The trichoderma koningiopsis disclosed by the invention can inhibit potato fungi alternaria solani, rhizoctonia solani, fusarium williamsii and fusarium solani, and can reduce occurrence of various fungal diseases such as potato early blight, fusarium wilt and black scurf.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma antagonizing various potato pathogenic fungi and applications thereof. Background technique [0002] Potato is the fourth largest food crop after wheat, rice and corn in the world, and my country is the largest potato producer in the world. As the main grain, vegetable and processing raw material crop, potato is widely planted in our country. Potatoes are rich in nutrients, so they are often troubled by diseases during potato planting, especially long-term continuous cropping causes a large number of pests and diseases to gather and spread, which greatly limits the potential for increasing potato production, and also greatly increases potato storage losses. Among the diseases that occur widely in my country, fungal hazards are the main ones, and the main fungal diseases include late blight, early blight, black mole, and Fusarium wilt. [0003] Potato early ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38
Inventor 魏琪郭梅邵洪涛吕军王文重杨帅董学志毛彦芝闵凡祥张抒
Owner 黑龙江省农业科学院马铃薯研究所