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Composite microbial agent for potato black scurf

A compound microbial inoculum and a technology for potato black nevus, which is applied in the field of agricultural microorganisms, can solve problems such as unstable control effect, and achieve the effects of promoting utilization rate, adjusting soil flora balance, and improving quality.

Active Publication Date: 2021-03-12
DEZHOU YUANHE AGRI TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the control effect of most microbial agents is not stable at present.

Method used

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  • Composite microbial agent for potato black scurf
  • Composite microbial agent for potato black scurf
  • Composite microbial agent for potato black scurf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of the composite microbial inoculant of preventing and treating potato black mole, the steps are as follows:

[0040] (1) Inoculate Bacillus atrophaeus CICC 20238 in LB liquid medium, and culture it in a shaker flask for 36 hours at 37°C to obtain liquid seeds, inoculate the obtained liquid seeds in liquid fermentation medium A, and adjust the pH value to 7.0, the aeration ratio is 1:1, the rotating speed is 170 rev / min, cultivated at 37°C for 36h to obtain a fermented liquid, and the fermented liquid is centrifuged and dried to obtain an effective viable count of 5 billion / g of solid microbial agent A;

[0041] (2) Bacillus polymyxa ACCC 10122 was inoculated in LB liquid medium, and cultured in shake flasks for 40 hours at 35°C to obtain liquid seeds, which were inoculated into liquid fermentation medium B, and regulated The pH value is 7.0, the ventilation ratio is 1:0.75, and the rotation speed is 150 rpm. Cultivate at 35°C for 24 hours to ob...

Embodiment 2

[0052] Adopt the method as described in embodiment 1, difference is, by weight, 7 parts of solid microbial agent A, 20 parts of solid microbial agent B, 30 parts of solid microbial agent C are mixed in step (4).

[0053] In the prepared composite microbial agent for preventing and treating potato black mole, each gram of the composite microbial agent contains: Bacillus atrophaeus CICC 20238 effective viable count 600 million / g, Bacillus polymyxa ACCC 10122 effective viable count 17 100 million / g, Trichoderma harzianum ACCC 33109 effective viable count is 1.5 billion / g, total effective count is 3.8 billion / g.

Embodiment 3

[0055] Adopt the method as described in embodiment 1, difference is, in step (4), 10 parts of solid microbial agent A, 20 parts of solid microbial agent B, 30 parts of solid microbial agent C are mixed by weight.

[0056] In the prepared composite microbial agent for preventing and treating potato black mole, each gram of the composite microbial agent contains: Bacillus atrophaeus CICC 20238 effective viable count 800 million / g, Bacillus polymyxa ACCC 10122 effective viable count 16 100 million / g, Trichoderma harzianum ACCC 33109 effective viable count 1.5 billion / g, total effective count 3.9 billion / g.

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Abstract

The invention relates to a compound microbial agent for potato black scurf. The microbial agent comprises bacillus atrophaeus CICC 20238, Paenibacillus polymyxa ACCC 10122 and Trichoderma harzianum ACCC 33109. The invention also relates to a preparation method of the compound microbial agent. The compound microbial agent provided by the invention has an excellent prevention and treatment effect onpotato black scurf, effectively reduces the morbidity of potato black scurf, and has a certain growth promoting effect.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a compound microbial bacterial agent for potato black mole disease. Background technique [0002] Potato is one of the four major crops in my country. Its tubers are rich in protein and starch, and are edible and can be used in industrial production. Potato planting areas are extensive and expanding year by year, and soil-borne diseases of heavy cropping are more common. Among them, potato black mole disease is becoming more and more serious and common. Potato black mole has a serious impact on potato yield and quality. [0003] Potato black mole (also known as stem canker, stalk rot, rhizoctonia canker and black rough skin disease) is caused by Rhizoctonia solani and is a fungal disease mainly on diseased seed potatoes and soil way for spreading. Pathogens mainly harm young shoots, stem bases and stem tubers. It can cause symptoms such as bud ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/38A01P3/00C12R1/12C12R1/07C12R1/885
CPCC12N1/20C12N1/14A01N63/22A01N63/38
Inventor 展彬王金涛周自强杜绍国
Owner DEZHOU YUANHE AGRI TECH DEVCO
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