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Application of liquidambaric lactone in treatment of cancers

A Lulutong, purpose technology, applied in the field of medicine, can solve the problems of easy recurrence, heavy medical treatment, economic burden, and inability to guarantee the recovery of patients.

Pending Publication Date: 2021-03-23
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high incidence of colorectal cancer has caused heavy medical and economic burdens
The main treatment methods for colorectal cancer are surgery, radiotherapy with the help of equipment, and adjuvant chemotherapy with chemical drugs. However, even if these thre

Method used

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  • Application of liquidambaric lactone in treatment of cancers
  • Application of liquidambaric lactone in treatment of cancers
  • Application of liquidambaric lactone in treatment of cancers

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0087] Experimental Example 1. Inhibition of the compound represented by formula I on tumor cell proliferation involving the Wnt / β-catenin signaling pathway

[0088] Experimental materials: HCT116 cells, HCC827 cells, H460 cells, PC-3 cells, NCL-N87 cells, U-2OS cells, SK-OV3 cells, HEPG2 cells, MDA-MB-231 cells were purchased from the Cell Bank of the Chinese Academy of Sciences; H1975 cells , ASPC1 cells, PANC1 cells, CFPAC1 cells, BXPC3 cells, and SW480 cells were purchased from ATCC; the compound represented by formula I was purchased from the National Center for Standard Materials; 4% paraformaldehyde and crystal violet were purchased from Biyuntian.

[0089] Experimental procedure: cells were inoculated into a 6-well plate (1500 cells / well), and after incubation for 24 hours, the compound represented by formula I (0, 5, 10, 20, 40 μM) was administered. After 14 days of treatment, cells were washed 3 times with PBS, fixed with 4% paraformaldehyde for 10 min, paraformaldeh...

experiment example 2

[0091] Experimental Example 2. Effects of Compounds Shown in Formula I on Colorectal Cancer Cell (HCT116) Subcutaneous Transplanted Tumors

[0092] Experimental materials: BALB / c nude mice (four weeks old), male, purchased from Shanghai Xipuer-Bicai Laboratory Animal Co., Ltd. BALB / c nude mice were raised under SPF conditions.

[0093] Solution preparation: water is used as a solvent, and the compound represented by formula I is dissolved to prepare a suspension.

[0094] Cell culture: Observe the state of HCT116 cells every day. When the cells are in good condition, that is, in the logarithmic growth phase, digest and collect the cells, add 1mL PBS, carefully wash the cell pellet twice, resuspend the cell pellet in PBS, and adjust the cell density. Adjusted to 5×10 6 individual / mL.

[0095] Subcutaneous injection of nude mice: take the nude mice to be vaccinated, wipe the injection site (right shoulder blade) of the nude mice with alcohol cotton, blow the cell suspension a...

experiment example 3

[0100] Experimental Example 3. The Wnt / β-catenin pathway inhibitory effect of the compound shown in formula I

[0101] Experimental materials: 293 cells were purchased from the Cell Bank of the Chinese Academy of Sciences. AB line zebrafish were purchased from the National Zebrafish Resource Center.

[0102] TOP / Flash experimental procedure: 293FT cells were inoculated into 96-well white plate (20000 cells / well), 24 hours after seeding the plate, given transfection TOP / Flash plasmid and Renilla plasmid, and 6 hours later, Passepartout lactone (0, 5, 10, 20 μM ). After 24 hours of treatment, the activity of luciferase was detected.

[0103] Zebrafish embryo "eye loss" experimental procedure: when the AB line zebrafish embryo was 6hpf, grouped according to different dosing groups and administered corresponding compounds at the same time. Among them, the model group was given 6BIO (1 μM), and the compound group shown in formula I was given the compound shown in formula I (10 μ...

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Abstract

The invention finds that a compound shown in the formula I obtained in the traditional Chinese medicine fructus liquidambaris shows a targeted inhibition effect on a Wnt signal path. Therefore, the compound shown in the formula I can be developed into a brand-new Wnt inhibitor and a medicine for treating cancers, especially colorectal cancers, so that a new direction is opened up for developing anti-tumour medicines derived from traditional Chinese medicines.

Description

technical field [0001] The present invention relates to the field of medicine. Specifically, the present invention relates to the application of paretolide in the treatment of cancer, especially colorectal cancer. Background technique [0002] Colorectal cancer is the third most common malignant tumor in the world, and its mortality rate is the fourth. In the past 40 years, colorectal cancer has become the third most common malignant tumor in my country, accounting for 10.30% of all malignant tumors. The high incidence of colorectal cancer has caused a heavy medical and economic burden. The main treatment methods for colorectal cancer are surgery, radiotherapy with the help of equipment, and adjuvant chemotherapy with chemical drugs. However, even if these three methods are combined, the patient's recovery cannot be guaranteed, and postoperative recurrence is easy. And related studies have shown that in some developed countries, the five-year survival rate of colon cancer...

Claims

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Application Information

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IPC IPC(8): A61K31/585A61K36/185A61P35/00A61P35/04A61P25/00A61P19/08A61P17/00A61P9/00A61P43/00A61P3/00A23L33/105
CPCA23V2002/00A61K31/585A61K36/185A23L33/105A61P3/00A61P9/00A61P17/00A61P19/08A61P25/00A61P35/00A61P35/04A61P43/00A23V2200/308A23V2250/21
Inventor 屈祎闫蓉柯细松张卫东黄飘
Owner SHANGHAI UNIV OF T C M
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