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Method for determining concentration of prucalopride in blood plasma by liquid chromatography-mass spectrometry

A technology of prucalopride and liquid mass spectrometry, which is applied in the field of medicine, can solve the problems of speed, precision, sensitivity, and selectivity to be improved, and achieve the effect of simple pretreatment method, strong specificity, and high sensitivity

Inactive Publication Date: 2021-04-09
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the speed, precision, sensitivity and selectivity of the

Method used

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  • Method for determining concentration of prucalopride in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of prucalopride in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of prucalopride in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031]Example: Human K2Determination of Pully Caspel Concentration in EDTA Plasma

[0032]First, experimental materials and analysis equipment

[0033]Pusuka Buli (analyte): Hefei Xinfeng Technology Co., Ltd. or the same, higher level of standard

[0034]Pully Kay Buli -13C-D3(Internal standard): TLC Pharmaceutical Standards or the same, higher level of standard

[0035]See Table 1 below using the reagent:

[0036]Table 1 reagent detail

[0037]

[0038]

[0039]Note: The same level or higher reagent can also be used.

[0040]See Table 2 using the analytical equipment:

[0041]Table 2 Using equipment details

[0042] Assembly model manufacturer BinaryPump (binary pump) ACPUMP Absciex Degasser (degasser) Degasser Absciex Columnoven (constant temperature column) Accolumnoven Absciex Autosampler (Auto Sample) Acautosampler Absciex Samplerack (sample rack) Rackchanger Absciex MassSpectrometer (mass spectrometer) Triplequad TM 6500+

[0043]The same LC / MS / MS system can also be used.

[0044]Second, liquid condi...

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PUM

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Abstract

The invention discloses a method for determining the concentration of prucalopride in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination, and comprises the following steps: taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, separating by a chromatographic column, and detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of prucalopride; and according to the method, the linear range of the plasma standard curve is 0.1-10ng/mL, the intra-batch precision RSD and the inter-batch precision RSD are both smaller than +/-15%, and the method is suitable for measuring the concentration of prucalopride in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining a drug, in particular to a method for determining the concentration of prucalopride in plasma by liquid mass spectrometry. Background technique [0002] As a new generation of highly selective and high affinity serotonin 4 (5-HT4) receptor agonist, prucalopride has substantially improved chronic constipation ( CC) patients have brought obvious benefits. Prucalopride specifically and selectively stimulates intestinal 5-HT4 receptors, stimulates the intestinal tract to produce high-amplitude propulsive contraction, promotes the peristalsis of the intestinal tract (especially the colon), and improves intestinal motility and remodeling. It has a significant effect on defecation function. A large amount of evidence-based evidence has fully confirmed that prucalopride is effective in the treatment of CC. It can improve intestinal motility and re...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范志鸿
Owner 徐州立顺康达医药科技有限公司
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