Indole alkaloid compounds in Noni enzyme and its preparation method and application

A technology of indole alkaloid and noni enzyme, applied in the field of phytochemistry, can solve problems such as lack of noni enzyme medicinal development, and achieve the effects of inhibiting α-glucosidase activity, wide source of raw materials and simple preparation process

Active Publication Date: 2021-12-28
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a lack of medicinal development of Noni enzyme

Method used

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  • Indole alkaloid compounds in Noni enzyme and its preparation method and application
  • Indole alkaloid compounds in Noni enzyme and its preparation method and application
  • Indole alkaloid compounds in Noni enzyme and its preparation method and application

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preparation example Construction

[0026] The Noni enzyme used in the present invention is the commercially available Noni enzyme produced by Hainan Dazhou Island Noni Food Co., Ltd. Its preparation method comprises the following steps:

[0027] (1) wash the epidermis of ripe noni fruit with a brush;

[0028] (2) Wash and drain the bottle, knife and cutting board for later use;

[0029] (3) Take out the noni fruit, drain the water, and slice it; put all the sliced ​​noni fruit into a clean fermentation bottle according to the ratio of 1:3:10 (1 catties of sugar: 3 catties of noni fruit: 10 catties of water) (20L white plastic bucket). About 20% of the space is left in the bottle for fermentation;

[0030] (4) Close the bottle cap tightly and paste the production date;

[0031] (5) During the fermentation period of noni fruit, open and stir or shake the bottle every day in the first month, so that the fruit on the top layer that is not soaked in the liquid can penetrate; in order to prevent the fermentation o...

Embodiment 1

[0034] The preparation method of indole alkaloid compound in noni ferment comprises the following steps:

[0035] Mix noni enzyme and ethyl acetate at a volume ratio of 1:1 and extract to obtain an organic phase and an aqueous phase. After the aqueous phase is concentrated, column chromatography, thin layer chromatography, and molecular sieve chromatography are performed sequentially; column chromatography The conditions are: the chromatographic column is a macroporous resin D201 column, and the ethanol-water mixed solvent with a volume percentage of ethanol of 20% is used as an eluent; the thin-layer chromatography condition is: a methanol-chloroform mixture with a volume percentage of methanol of 20% The solvent is a developer; the molecular sieve chromatography conditions are as follows: the molecular sieve is Sephadex LH-20, and a chloroform-methanol mixed solvent with a volume percentage of chloroform of 50% is used as an eluent.

[0036] Obtain indole alkaloid compounds,...

Embodiment 2

[0046] The preparation method of indole alkaloid compound in noni ferment comprises the following steps:

[0047] Mix noni enzyme and ethyl acetate at a volume ratio of 1:0.8 and extract to obtain an organic phase and an aqueous phase. After the aqueous phase is concentrated, it is subjected to column chromatography, thin layer chromatography, and molecular sieve chromatography; column chromatography The conditions are: the chromatographic column is a macroporous resin D201 column, and the ethanol-water mixed solvent with a volume percentage of ethanol of 30% is used as an eluent; The solvent is a developer; the molecular sieve chromatography conditions are as follows: the molecular sieve is Sephadex LH-20, and a chloroform-methanol mixed solvent with a volume percentage of 1% of chloroform is used as an eluent.

[0048] Obtain the indole alkaloid compound in 2 noni enzymes, consistent with embodiment 1 through detection.

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Abstract

The present invention provides the indole alkaloid compound in Noni enzyme, and its structural formula is as shown in the following formula: The present invention also provides the preparation method of the above-mentioned compound and the indole alkaloid compound in Noni enzyme in the preparation of α-glucosidase application of inhibitors. The preparation process of the indole alkaloid compound in the noni enzyme provided by the invention is simple, the cost is low, and the source of raw materials is wide.

Description

technical field [0001] The invention belongs to the field of phytochemistry, and relates to an indole alkaloid compound in noni enzyme, a preparation method of the compound, and an application of the indole alkaloid compound in noni enzyme. Background technique [0002] Alkaloids are a type of nitrogen-containing basic organic compounds that exist in nature (mainly plants, but some also exist in animals). They have alkali-like properties, so they were also called pseudoalkali in the past. Most of them have a complex ring structure, and nitrogen is mostly contained in the ring, which has significant biological activity and is one of the important active ingredients in Chinese herbal medicine. [0003] Many alkaloids can cure diseases. For example, berberine in the rhizome of Coptis chinensis is the main component of berberine, which has antibacterial and anti-inflammatory effects, and is very effective for acute and chronic inflammation of the gastrointestinal tract. Some al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/04A61P3/10
CPCC07D471/04A61P3/10C07B2200/07
Inventor 陈光英张斌李小宝赵婷黄柯雷王浩
Owner HAINAN NORMAL UNIV
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