Large-field high-throughput flow cytometric analysis system and analysis method

Abstract

The invention relates to a large-field-of-view high-throughput flow cytometry analysis system and an analysis method. The analysis system includes: an illumination module for generating monochromatic light; a cell sample fixing control module for fixing and adjusting the position of the cell sample , so that the cell sample is located at the position of the imaging field of view, and at the same time control the uniform flow of the cell sample; the data acquisition module is used to collect the interference image formed after the monochromatic light passes through the cell sample; the control processing module is used to process the interference image to obtain The virtual focal length is used to reconstruct the interference image according to the virtual focal length to obtain the reconstructed image. Based on the pre-trained cell segmentation and classification model, the reconstructed image is analyzed to obtain the analysis result of the cell sample. The large-field-of-view high-throughput flow cytometric analysis system of the present invention provides a large-field, label-free cell phase image through computational microscopic imaging, and the sample control part is no longer limited by the imaging field of view.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a large-field high-throughput flow cytometry analysis system and an analysis method. Background technique [0002] Cell analysis is an important means of medical detection. The detection methods of existing cell analyzers include manual method, impedance method, flow cytometry and image analysis methods. The manual method is to stain the cells and settle them on the glass counting plate. The doctor observes and counts the types of cells with the help of a microscope. The impedance method includes the DC impedance method and the AC impedance method. The DC impedance method can detect the size of the cells and the AC impedance. The method can carry out real-time label-free data analysis; flow cytometry is a combination of fluid mechanics theory and biomedical knowledge, usually using sheath flow technology, wrapping cells with sheath flow and passing through t...

AI Technical Summary

Problems solved by technology

[0003] The detection methods of existing cell analyzers still have the following deficiencies: (1) the structure of traditional detection instruments is relatively complicated, the cost is high, and the scope of application is limited; (2) traditional detection instruments generally need to use dyeing methods such as fluorescence and contrast agents, The steps are cumbersome and will damage the cells; (3) The existing imaging flow cytometer requires a focusing device, the imaging field of view is small, and the cell flux is limited to a single channel

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