Biomarker group and application thereof

A technology of biomarkers and male reproduction, applied in the biomarker group and its application field, can solve the problem of undetected prochloraz dosage and other issues

Inactive Publication Date: 2021-05-18
HUNAN UNIV OF HUMANITIES SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are many studies in this field of research on chemical pesticides that affect human health by exposing experimental animals to these harmfu

Method used

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  • Biomarker group and application thereof
  • Biomarker group and application thereof
  • Biomarker group and application thereof

Examples

Experimental program
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Embodiment 1

[0036] 1. Materials and methods

[0037] 1. Animals and experimental design

[0038] First, Kunming sexually mature mice (6-10 weeks old) with a body weight of (25±2) g and a male-to-female ratio of 2:1 were selected and purchased from Hunan Slack Jingda Experimental Animal Co., Ltd. All animals were housed in a laboratory controlled environment (25°C, 50% humidity and light:dark cycle 12h:12h) while allowing free access to food and drinking water. On the next morning, female mice were checked for the presence of vaginal suppositories. Vaginal secretions from female mice without vaginal suppositories were taken from cotton swabs soaked in saline, and applied to slides containing a drop of saline. Examine the sperm under a low power microscope. The date the vaginal suppository or sperm is found is day 0 of pregnancy.

[0039] Mice were mated in cages with a male-to-female ratio of 2:1, and the fertilized female mice were randomly divided into 3 groups: control group (MA; pr...

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Abstract

The invention discloses a biomarker group and application thereof, and belongs to the technical field of bioengineering. By applying an RNA-seq technology, the result shows that the mRNA expression level of an mt-Nd6 gene in a male reproductive system is lowered, and meanwhile the mRNA expression level of an Slc12a4 gene in the male reproductive system is raised; and therefore, the Slc12a4 gene and the mt-Nd6 gene can serve as the biomarker group for diagnosing male reproductive toxicity caused by prochloraz. By studying the mechanism that prochloraz affects the male reproductive system, the invention provides the biomarker group for diagnosing the male reproductive toxicity caused by prochloraz, the Slc12a4 gene and the mt-Nd6 gene can provide an important basis for preventing and treating male reproductive diseases caused by prochloraz for human, and the study has practical significance.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a biomarker group and its application. Background technique [0002] With the extensive use of pesticides in agriculture, the adverse effects on both human reproductive health and wild animal reproductive capacity have increased. There are quite a lot of reports on the adverse effects of pesticides on the reproductive ability of wild animals and plants, but there are few reports on human exposure to chemicals and human reproductive health effects (such as deformed sex organs and poor sperm quality, etc.). However, recent studies have shown a causal relationship between human exposure to pesticides and poor sperm quality in men, altered testicular epigenetic expression in male health, and the incidence of cryptorchidism. It is well known that many chemical pesticides have been experimentally proven to affect important endocrine processes in humans, and some of these chemic...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/142C12Q2600/158
Inventor 胡军和董锦熠唐涛
Owner HUNAN UNIV OF HUMANITIES SCI & TECH
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