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A system and method of performing a biological experiment with adaptive cybernetic control of procedural conditions

A technology of biological experiments and conditions, applied in the field of biological experiments, can solve problems such as changes in results

Pending Publication Date: 2021-05-28
阿克托里斯有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Experiments performed in limited facilities can be performed with great care, but due to changes in temperature, humidity, pH, sample volume, particle concentration, gas concentration, exposure to light, cross-contamination from other experiments, viruses from ambient air, and Bacterial contamination, and aerosol contamination from other laboratory reagents and antimicrobials, and / or other factors, with variable results

Method used

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  • A system and method of performing a biological experiment with adaptive cybernetic control of procedural conditions
  • A system and method of performing a biological experiment with adaptive cybernetic control of procedural conditions
  • A system and method of performing a biological experiment with adaptive cybernetic control of procedural conditions

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Embodiment approach

[0073] To further illustrate exemplary embodiments of the present disclosure, factors generally considered to affect the outcome of individual procedures, and thus of biological experiments, including those in cellular and molecular biology, can be divided into two categories.

[0074] The first category includes physical and chemical conditions that can be measured and controlled by physical means, such as temperature, humidity, O 2 and CO 2 concentration, as well as the pH and concentration of nutrients, electrolytes, and reagents in the container. In the case of the first group, when a deviation from the regulation observed in one of these conditions is found, said deviation can be corrected by physical changes immediately. For example, water can be added to the experimental vessel to compensate for fluid loss due to low humidity. In the second group of cases, a deviation from one of these conditions may require a physical change followed by some duration for the change t...

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Abstract

A method for performing a biological experiment includes accessing a protocol that specifies operations and prescribes a plurality of conditions for carrying out the biological experiment in a defined space. The method includes performing the sequence of operations according to the protocol; and periodically before or as the sequence of operations are performed, obtaining observations of particular conditions of the plurality, and comparing the particular conditions as observed to the particular conditions as prescribed. When a particular condition as observed deviates from the as prescribed by more than a predetermined threshold for the particular condition, the method includes interrupting the sequence of operations, accessing a corrective protocol that specifies an operation for remediating the particular condition as observed to within the predetermined threshold of the particular condition as prescribed, performing the operation to remediate the particular condition according to the corrective protocol, and resuming the sequence of operations.

Description

technical field [0001] The present disclosure relates generally to biological experiments, and in particular to adaptive cybernetic control of biological experiments utilizing procedural conditions. Background technique [0002] Biomedical research covers a wide range of fields. Today, one of the most important studies is the study of cellular and molecular biology and diseases originating at the cellular and molecular level. [0003] Cellular and molecular biology experiments can often be characterized as a series of steps beginning with the preparation of a culture of a particular cell type in a suitable growth medium. Portions of the cell culture and growth medium are then dispensed into experimental vessels (eg, flasks, test tubes, or well plates), allowed to grow for a period of time (perhaps in an incubator, bioreactor, or fermenter), and the cells observed or measured Conditions of the culture (eg, photomicrographs or cell counts). Treatment of a cell culture with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36
CPCC12M41/48G01N35/00613G01N35/0099G01N2035/00277
Inventor M-I·比特纳T·A·弗莱明A·P·罗沃斯
Owner 阿克托里斯有限公司
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