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Mesenchymal stem cell-derived exosomes based on Tongxinluo pretreatment and preparation method thereof

A technology of mesenchymal stem cells and exosomes, which is applied in the field of high-efficiency exosomes derived from mesenchymal stem cells and its preparation, can solve the problems of insignificant effects and achieve the effect of reducing the size of myocardial infarction and reducing post-myocardial infarction inflammation

Active Publication Date: 2022-02-01
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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Problems solved by technology

The paracrine function of MSCs is an important way for them to function (Ranganath, S.H., et al., Harnessing the mesenchymalstem cell secretome for the treatment of cardiovascular disease. Cell StemCell, 2012.10(3):p.244-58.), however Clinical studies have shown that although mesenchymal stem cells can improve cardiac function after infarction through paracrine protection to a certain extent, the effect is not significant

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  • Mesenchymal stem cell-derived exosomes based on Tongxinluo pretreatment and preparation method thereof
  • Mesenchymal stem cell-derived exosomes based on Tongxinluo pretreatment and preparation method thereof
  • Mesenchymal stem cell-derived exosomes based on Tongxinluo pretreatment and preparation method thereof

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Embodiment 1

[0033] Preparation method of exosomes derived from mesenchymal stem cells:

[0034] The preparation method of exosomes derived from mesenchymal stem cells: the primary rat (Sprague-Dawley rat, 60-80g) BM-MSCs were isolated by the differential attachment method, and the BM-MSCs were subcultured and expanded to the 3rd-4th generation for later use. BM-MSCs complete medium (IMDM, Gibco, USA) was pretreated with 400 μg / mL Tongxinluo for 24 hours, and the cell medium was replaced with IMDM partial medium (without adding fetal bovine serum) to continue culturing. After 48 hours, the conditioned medium was collected and ultracentrifugation was used to separate the exosomes secreted by BM-MSCs pretreated with Tongxinluo (MSC TXL -Exo). The specific steps of the ultracentrifugation method include: after collecting the conditioned medium, centrifuge at 300g for 10min to remove cells; centrifuge at 2000g for 20min to remove cell debris; high-speed centrifuge at 16500g for 30min to remov...

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Abstract

The invention provides a mesenchymal stem cell-derived exosome based on Tongxinluo pretreatment and a preparation method thereof. The present invention provides a method for preparing exosomes derived from mesenchymal stem cells that upregulates the expression level of miR-146a, the method comprising: using Tongxinluo to pretreat mesenchymal stem cells, and cultivating the treated mesenchymal stem cells to The secreted exosomes were collected. The present invention can prepare mesenchymal stem cell-derived exosomes with the ability to promote angiogenesis and / or myocardial repair.

Description

technical field [0001] The present invention relates to a mesenchymal stem cell-derived exosome based on drug pretreatment and its preparation method, in particular to a high-efficiency mesenchymal stem cell-derived exosome based on drug Tongxinluo pretreatment and its preparation method. Background technique [0002] WHO statistics show that in 2016, cardiovascular disease was the leading cause of death in the world (about 17.6 million people, 32.2%). Although the current medical level has been greatly improved, acute myocardial infarction (acute myocardial infarction, AMI) is still the leading cause of death worldwide. One of the main causes of disability (GBD 2016 Mortality Collaborators. Global, regional, and national under-5 mortality, adult mortality, age-specific mortality, and life expectancy, 1970-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017 Sep 16; 390(10100):1084-1150; Roth, G.A., et al., Global, Regional, and National Bur...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K35/28A61P9/10
CPCC12N5/0662C12N5/0663C12N5/0667A61K35/28A61P9/10C12N2509/00C12N2500/76C12N2500/82
Inventor 杨跃进熊语嫣汤瑞杰
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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