Sterile cell sampling device and method based on injector

A sampling device and syringe technology, applied in the direction of sterilization methods, biological material sampling methods, and methods of supporting/immobilizing microorganisms, etc., can solve the problems of cumbersome operation, cover contamination, uncontrollable sampling, etc., and achieve simple operation and avoid Waste, the effect of precisely controlling the amount of sampling

Pending Publication Date: 2021-10-08
SHENZHEN CELLBRI BIO INNOVATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation of the existing sampling device is cumbersome, the sampling is uncontrollable, and frequent opening of the cover is easy to cause pollution

Method used

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  • Sterile cell sampling device and method based on injector
  • Sterile cell sampling device and method based on injector
  • Sterile cell sampling device and method based on injector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] like figure 1 and figure 2 As shown in the structure, the embodiment of the present invention provides a sterile cell sampling device based on a syringe 3, which includes a sampling container 1, a sterile filter 2, a syringe 3, a control valve 4, and a first conduit 5 and the second conduit 6;

[0036] The interior of the sampling container 1 has an accommodating cavity for accommodating the cell fluid, and the top is provided with a first interface 11 and a second interface 12 communicating with the accommodating cavity for realizing the maximum sampling capacity of the sampling container 1; like figure 1 As shown in the structure, the first interface 11 is arranged on the top left side of the sampling container 1, the second interface 12 is arranged on the top right side of the sampling container 1, and the first interface 11 and the second interface 12 are arranged oppositely; as figure 2 As shown in the structure, the first interface 11 is arranged on the top l...

Embodiment 2

[0046] The embodiment of the present invention also provides a cell sampling method using the above-mentioned sterile cell sampling device. The fluid driving force is formed by pulling the negative pressure generated by the syringe 3 to introduce the cell liquid into the sampling container 1. The cell sampling method includes The following steps:

[0047] Step S10, close the control valve 4 of the sterile cell sampling device to make it in a closed state, connect the sterile cell sampling device to the cell drug production device through the first conduit 5, and make the first interface 11 and the second interface 12 in the sampling state The highest position of container 1;

[0048] Step S20, open the control valve 4 of the sterile cell sampling device to make it in an open state, and pull the piston core rod 32 of the syringe 3 toward the outside of the syringe 31 to generate a negative pressure lower than atmospheric pressure in the sampling container 1, and then the Quant...

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PUM

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Abstract

The invention relates to a sterile cell sampling device and method based on an injector. The sterile cell sampling device comprises a sampling container, a sterile filter, the injector, a control valve, a first guide pipe and a second guide pipe; a first interface and a second interface are formed in the top of the sampling container; the first guide pipe is connected with the first interface and a cell drug production device; the second guide pipe is connected with the second interface and the sterile filter; the injector comprises a needle cylinder, a piston and a piston core rod; the needle cylinder communicates with the sterile filter; and the control valve is installed in the middle of the first guide pipe, has an open state and a closed state, and is used for controlling on-off of the first guide pipe. The sterile cell sampling device has the advantages that the sampling is simple and convenient, the sampling amount can be accurately controlled, the sterile sampling is effectively ensured, the probability that the cells are polluted is greatly reduced, and the waste of cell sap in the guide pipes can be avoided.

Description

technical field [0001] The invention relates to the technical field of drug sampling, in particular to a syringe-based aseptic cell sampling device and method. Background technique [0002] Since the production process of cell-based drugs has the characteristics of many preparation steps and complex processes, the complex process operation and the in vitro environment of cells have a significant impact on the biological activity of cells; therefore, regular cell sampling is required in the production process of cell-based drugs , to monitor indicators such as cell viability, loss rate, and density. When using the existing sampling device to take samples, the experimenter usually uses the aseptic sampling device to take samples. The existing aseptic sampling method is generally to move the device into a sterile environment, and open the cover for sampling. However, the operation of the existing sampling device is cumbersome, the sampling is uncontrollable, and frequent openi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/26C12M1/36C12M1/00C12Q1/24
CPCC12M33/07C12M33/06C12M37/02C12M23/28C12M23/22C12M41/00C12Q1/24
Inventor 郑伟武姚嘉林郭霄亮商院芳
Owner SHENZHEN CELLBRI BIO INNOVATION TECH CO LTD
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