Application of LINC01996 in diagnosis of allergic airway inflammation
A technology for airway inflammation and allergic rhinitis, applied in the field of biomedicine, can solve the problems of few biomarker research reports
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Embodiment 1
[0057] Example 1 Real Time PCR Detection of Changes in the Expression of Target Genes in Blood Samples of Patients with Allergic Rhinitis-Asthma Syndrome
[0058] 1. Purpose of the experiment
[0059] The SYBR GreenⅠReal Time PCR method was used to detect the changes of lncRNA transcription level of the target gene in the blood samples of patients with allergic rhinitis-asthma syndrome.
[0060] 2. Experimental materials
[0061] 1. Sample Checklist
[0062] In this study, 20 patients with allergic rhinitis-asthma syndrome and 20 normal subjects were recruited. The clinical information is shown in Table 1. Blood samples were collected for analysis and research.
[0063] Table 1 Basic clinical information of the people involved in this study
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[0065] 2. Experimental main reagents
[0066] Table 2 List of Reagents Used
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[0068] 3. The main instruments of the experiment
[0069] Table 3 list of equipment used
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[0072] 3. Expe...
Embodiment 2
[0127] Embodiment 2 The diagnostic efficacy verification of LINC01996
[0128] Receiver operating curve (ROC) was drawn by SPSS software, AUC value, sensitivity and specificity were analyzed, and the diagnostic efficacy of the indicators alone was judged.
[0129] As shown in Table 9, Figure 5 As shown, LINC01996 has high diagnostic efficiency (AUC value 0.858, sensitivity value 0.750, specificity value 0.850), suggesting that LINC01996 can be used to diagnose allergic rhinitis-asthma syndrome.
[0130] Table 9 The area under the ROC curve
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