Application of endogenous polypeptide in preparation of targeted medicine for preventing or treating endometrial cancer

An endometrial cancer, endogenous technology, applied in the field of medicine, can solve the problems such as the undisclosed drug mechanism of endometrial cancer, the unoptimistic prognosis, and the high metastasis rate of patients.

Active Publication Date: 2021-12-28
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although peptide research is still in its infancy, it has shown excellent therapeutic potential in many diseases: for example, PGPIPN inhibits the invasion and metastasis of ovarian cancer cells, and peptide drugs leuprolide and goserelin have begun to be used in the treatment of prostate cancer. , but the role of endogenous polypeptides in endometrial cancer is rarely reported. Considering that the types and quantities of endogenous polypeptides are far more than those of macromolecular proteins, from the perspective of endogenous polypeptides, it is very important for elucidating endometrial cancer. The regulatory effect of endogenous polypeptide molecules in the cancer microenvironment on endometrial cancer undoubtedly has important potential scientific value
[0006] Comprehensive treatment of endometrial cancer (surgical resection, radiotherapy and chemotherapy, endocrine therapy, targeted therapy, etc.) High metastasis rate, very poor prognosis
However, the potential mechanism of ECDP2 as a drug for the treatment of endometrial cancer has not yet been disclosed.

Method used

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  • Application of endogenous polypeptide in preparation of targeted medicine for preventing or treating endometrial cancer
  • Application of endogenous polypeptide in preparation of targeted medicine for preventing or treating endometrial cancer
  • Application of endogenous polypeptide in preparation of targeted medicine for preventing or treating endometrial cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The clinical samples collected were mainly from female patients who underwent surgery (Table 1). Six samples of cancer tissue from patients with endometrial cancer and 6 samples of uterine tissue from age-matched patients with benign uterine lesions were collected.

[0025] Table 1: Basic information of clinical samples

[0026] Endometrial cancer (n=6) Benign lesions (n=6) age 54.5±5.5 53.5±8.5 p＞0.05 BMI 23.4±1.2 23.1±4.1 p＞0.05

[0027] In Table 1, there was no statistical difference in age and BMI index between the two groups of patients (p>0.05).

[0028] The polypeptides derived from endometrial cancer tissue and normal endometrial epithelial tissue were extracted, and the expression profiles of polypeptides derived from endometrial cancer tissue and normal endometrial epithelial tissue were detected by i-TRAQ-labeled LC-MS / MS method. The results of mass spectrometry showed 873 differential polypeptides in the two groups of sa...

Embodiment 2

[0030] In order to screen suitable peptides for the purpose of research, we comprehensively consider: ① Higher expression abundance; ② Small differences within the group; ③ Good lipophilicity; ④ Conservation among species; , first screened out 2 polypeptides for preliminary research (Table 2). These two polypeptides are from the precursor protein LMNA and NOVA2 respectively. For the convenience of presentation and research, we named them ECDP1 / 2 (Endometrial Cancer Derived Peptide1 / 2, ECDP1 / 2) respectively. The amino acid sequence is shown in SEQ ID NO.1 to SEQ ID NO.2. Among them, ECDP1 was highly expressed in endometrial cancer tissue; ECDP2 was low expressed in endometrial cancer tissue.

[0031] Table 2: Physicochemical properties and species conservation analysis of target peptides

[0032]

[0033]

Embodiment 3

[0035] 1. Cell viability detection experiment (CCK-8 experiment)

[0036]Ishikawa cells used in this experiment were purchased from Jiangsu Kaiji Biotechnology Co., Ltd., and HEC-1-A cells were purchased from Shanghai Institute of Biological Cytology, Chinese Academy of Sciences. CCK8 reagent was purchased from Nanjing Novizan Biotechnology Co., Ltd., and RPMI-1640 medium was purchased from Jiangsu Kaiji Biotechnology Co., Ltd.

[0037] HEC-1-A and Ishikawa cells in the logarithmic growth phase were taken, counted after trypsinization, and seeded into 96-well plates at 5000 cells / well, with 6 auxiliary wells for each concentration. After 4-6 hours of cell attachment, add CCK8 detection solution (10 μL LCCK8 + 90 μL RPMI-1640 serum-free medium) to each well, and incubate for 2 hours to detect the absorbance value with a microplate reader, which is recorded as 0 hour. At the same time, the cells were treated with different concentrations of peptide ECDP2 working solutions (0ng / ...

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Abstract

The invention discloses application of endogenous polypeptide ECDP2 in preparation of a novel medicine for treating endometrial cancer. The amino acid sequence of the endogenous polypeptide ECDP2 is as shown in SEQ ID NO: 2. The invention also discloses a novel pharmaceutical composition for treating endometrial cancer. The polypeptide ECDP2 plays an important role in the malignant behavior mechanism of invasion and metastasis of endometrial cancer, and can become a specific marker for treating endometrial cancer, and the discovery can provide a basis for the development of new drugs for clinical diseases.

Description

[0001] Technical solutions [0002] The invention relates to the field of medicine, and relates to the application of endogenous polypeptides in the preparation of new therapeutic drugs for endometrial cancer. Background technique [0003] Endometrial cancer is one of the three major malignant tumors of the female reproductive tract. According to global cancer statistics, in the United States, the incidence of endometrial cancer ranks first among female malignant tumors, and one in 38 women is diagnosed with Endometrial cancer. In China, there are 63,400 new cases of endometrial cancer and 21,800 deaths each year. And in recent years, with the aging population, obesity and the widespread promotion of hormone replacement, the incidence of endometrial cancer has been increasing year by year and showing a younger trend, which seriously threatens the life and health of women. [0004] The pathogenesis of endometrial cancer is complex, and studies have shown that its mechanism in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K38/17A61P35/00A61P35/04
CPCC07K14/4703A61P35/00A61P35/04A61K38/00
Inventor 阮红杰寸云花王露瑶付子毅布沙来木·木哈买胡爽
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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