Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Amplicon library construction primer group suitable for single-ended sequencing and construction method

An amplicon library and primer set technology, which is applied in the field of amplicon library construction primer set and construction, can solve the problem that the amplicon library cannot meet the requirements of single-end sequencing, and achieves shortening TAT time, sequencing time, and operation. simple effect

Active Publication Date: 2022-01-07
臻和(北京)生物科技有限公司 +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on the problem that the amplicon library in the prior art cannot meet the requirements of single-end sequencing, the present invention has developed a primer set and library construction method for the amplicon library that can meet the requirements of single-end sequencing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amplicon library construction primer group suitable for single-ended sequencing and construction method
  • Amplicon library construction primer group suitable for single-ended sequencing and construction method
  • Amplicon library construction primer group suitable for single-ended sequencing and construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Taking 4 FFPEDNA samples (the sample numbers are DS0202, DS0203, DS0236 and DS0251 respectively, all of which are non-small cell lung cancer samples) as the detection objects, the amplicon libraries of the 4 samples were constructed using the primer set provided by the present invention, and a single End-to-end sequencing, the specific process is as follows:

[0102] 1. Primer panel design

[0103] (1) Design of multiple primers for the first round of PCR

[0104] For each target amplicon, design 2 pairs of multiplex primers, each pair of multiplex primers contains 1 multiplexed upstream primer and 1 multiplexed downstream primer;

[0105] Wherein, the first multiple upstream primers are the first adapter sequence and the target amplicon-specific primer sequence according to the 5'-3' direction, and the first multiple downstream primers are the second adapter sequence according to the 5'-3' direction and target amplicon downstream specific primer sequences;

[0106] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of high-throughput sequencing, in particular to an amplicon library construction primer group suitable for single-ended sequencing and a construction method. The primer group provided by the invention comprises a multiple primer pair of at least one target amplicon. Aiming at each target amplicon, the multiple primer pair comprises two pairs of multiple primers, and each pair of multiple primers comprises one multiple upstream primer and one multiple downstream primer. Each of the multiple upstream primer and the multiple downstream primer comprises a linker sequence and a target amplicon specific primer sequence. In the two pairs of multiple primers, the two multiple upstream primers contain different linker sequences, and the two multiple downstream primers contain different linker sequences. The primer group can be used for constructing an amplicon library suitable for single-ended sequencing, the sequencing time is effectively shortened, and the TAT time is shortened.

Description

technical field [0001] The invention relates to the technical field of high-throughput sequencing, in particular to an amplicon library construction primer set and construction method suitable for single-end sequencing. Background technique [0002] Amplicon library construction is a library construction method that adds adapters to both ends of the DNA fragments to be tested through PCR reactions. Only two rounds of PCR and two steps of purification are required to obtain the library of the target region. The first step is to contain the target region-specific The primers of the sex-binding sequence bind to the target region, and the second step is to connect the sequencing adapter through the PCR reaction, so that the constructed library can be used for on-machine sequencing. Compared with the capture library construction method, the amplicon library construction completes the library construction by customizing the primer panel. In clinical applications, the method of am...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C40B50/06C12N15/11C12Q1/6869
CPCC40B50/06C12Q1/6869C12Q2531/113C12Q2537/143C12Q2535/122
Inventor 李凤明魏丽许青陈维之杜波
Owner 臻和(北京)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com