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Urine blood coagulation factor IX and application of polypeptide fragment thereof in burns

A technology of coagulation factor and polypeptide fragment, which is applied to the application field of urine coagulation factor IX and its polypeptide fragment in burns, can solve the problems of poor skin integrity, aggravate the pain of patients, etc., and achieve the effect of convenient storage

Pending Publication Date: 2022-03-01
张曼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the integrity of the skin of patients with severe burns is poor. As an invasive skin test, there are many difficulties in the clinical application of blood tests for such patients. Repeated blood testing will also aggravate the suffering of patients.

Method used

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  • Urine blood coagulation factor IX and application of polypeptide fragment thereof in burns
  • Urine blood coagulation factor IX and application of polypeptide fragment thereof in burns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Collection and processing of urine specimens

[0021] The burn patients were selected as the burn group, and the healthy subjects who underwent physical examination during the same period were selected as the normal control group. Collect 30ml of fresh morning urine samples from the research subjects in each group after admission, and collect the urine in the catheter in the morning for those who cannot urinate normally, and place them in a dry and clean container. After the collected urine samples were centrifuged at 4000r / min for 5min, the supernatant was aspirated, divided into 2ml tubes, and stored in a -80°C refrigerator.

Embodiment 2

[0022] Example 2 Mass Spectrometry and Screening of Urine Peptides

[0023] Extract protein from urine samples and determine the concentration of the extracted protein. Mass spectrometric analysis of urine samples was performed by an OrbitrapFusion type mass spectrometer. Quantitative calculation was performed on the data obtained in the mass spectrometer of the experimental group and the normal control group. The difference between groups was analyzed by t-test, and the difference in protein expression was more than 1.5 times and the statistical test P<0.05 was used as the reference standard to screen for differentially expressed proteins.

Embodiment 3

[0024] Example 3 Identification and Analysis of Differential Peptides

[0025] The database used was Uniprot_Homo database, and the original mass spectrum files were processed by MaxQuant software, and the search parameter settings are shown in Table 1.

[0026]

[0027] Compared with healthy people, blood coagulation factor IX was highly expressed in the urine of burn patients, and its content in the urine of healthy control group and burn group was as follows: figure 1 As shown, there was a significant difference in the expression of blood coagulation factor IX in the urine of the normal control group and the burn group, and it increased with the aggravation of the degree of burn.

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Abstract

The invention provides application of a urine coagulation factor IX and a polypeptide fragment of the urine coagulation factor IX, and particularly relates to application of the urine coagulation factor IX and the polypeptide fragment of the urine coagulation factor IX in preparation of preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. Burns are common important wounds in daily life, about 5000-100000 people in every one million people are burnt every year, according to statistics of World Health Organization, more than 300,000 people die from burn patients globally every year, and the treatment survival rate of serious burns is still at a lower level. Researches prove that compared with healthy people (a normal control group), the expression of the urine blood coagulation factor IX and the polypeptide fragment thereof in burn patients is increased, and the content of the urine blood coagulation factor IX and the polypeptide fragment thereof is gradually increased along with the aggravation of the burn degree. The method can be used for various purposes of application detection of burn patients. The advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the urine blood coagulation factor IX and the polypeptide fragment thereof are detected by utilizing the urine sample.

Description

technical field [0001] The present invention relates to new uses of urine coagulation factor IX and its polypeptide fragments, in particular to the use of urine coagulation factor IX and its polypeptide fragments in burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation and mechanism applications for research, etc. Background technique [0002] Burn refers to the damage of human skin, tissues and even deep organs caused by chemical substances such as flame, high temperature gas, hot solid or liquid, radiation, electric energy, strong acid and strong alkali, and is a systemic comprehensive disease. Massive necrosis of wound tissue, infection, shock, and coagulation dysfunction after burns can cause a series of pathophysiological changes in the body. Different degrees of burns have different effects on the human body. Severe burns can cause damage to the internal environment of the human body, cau...

Claims

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Application Information

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IPC IPC(8): G01N33/86G01N33/68G01N33/543G01N27/62C12Q1/6883
CPCG01N33/86G01N33/6893G01N33/54306G01N33/6848C12Q1/6883C12Q2600/158G01N2800/52G01N2800/40G01N2333/745
Inventor 张曼王佶图
Owner 张曼
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