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Marker combination for predicting curative effect of gastrointestinal tumor immune checkpoint inhibitor and application thereof

A technology of immune checkpoints and inhibitors, applied in the detection/testing of microorganisms, genomics, proteomics, etc.

Pending Publication Date: 2022-03-25
GENE CRAB BIOTECH CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, while the aforementioned biomarkers are applicable to non-small cell lung cancer or melanoma, little is known about markers in gastrointestinal cancers that can be used to predict response or resistance in patients receiving immune checkpoint inhibitor therapy, and there is an urgent need. Effective predictive markers to aid clinical decision-making in immunotherapy for patients with gastrointestinal cancers

Method used

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  • Marker combination for predicting curative effect of gastrointestinal tumor immune checkpoint inhibitor and application thereof
  • Marker combination for predicting curative effect of gastrointestinal tumor immune checkpoint inhibitor and application thereof
  • Marker combination for predicting curative effect of gastrointestinal tumor immune checkpoint inhibitor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1 Effect of HLA-B62 supertype on the efficacy of immune checkpoint inhibitor therapy in patients with gastric gland tumors

[0116] This example is based on 36 patients with advanced gastric cancer who were treated with immune checkpoint inhibitors (PD-1 / PD-L1 inhibitors alone or in combination with CTLA-4 inhibitors), by evaluating that the patients carry germline HLA - Whether or not the supertype allele of B62 can predict the curative effect of its immunotherapy, the operation procedure of this experiment is as follows Figure 4 shown. The specific experiment content is as follows:

[0117] 1.1 Experimental operation

[0118] 1) Collect tumor tissue samples and peripheral blood samples from 36 patients with advanced gastric cancer before immunotherapy. The peripheral blood samples were separated by two-step centrifugation to obtain blood cell samples. Embedded (FFPE) tissue samples were drawn into the lysate with a disposable scalpel.

[0119] 2) Extracti...

Embodiment 2

[0127] Example 2 Effect of HLA-B62 supertype and TP53 gene mutation on the curative effect of immune checkpoint inhibitor therapy in patients with gastrointestinal tumors

[0128] Based on comparison of 91 patients with advanced gastrointestinal cancers treated with immune checkpoint inhibitors (PD-1 / PD-L1 inhibitors alone or in combination with CTLA-4 inhibitors) with or without germline HLA-B62 Effect of overtype allele combined with presence or absence of somatic mutation of TP53 gene in tumor tissue on treatment outcome. The specific experiment content is as follows:

[0129] 2.1 Experimental steps

[0130] 1) Collect tumor tissue samples and peripheral blood samples from 91 patients with advanced gastrointestinal cancer before immunotherapy. The peripheral blood samples are separated by two-step centrifugation to obtain blood cell samples. The fresh tumor tissue samples are crushed and processed into a cell suspension. Paraffin-embedded (FFPE) tissue samples were drawn ...

Embodiment 3

[0139] Example 3 Effect of HLA-B62 supertype and tumor mutation burden on the therapeutic effect of immune checkpoint inhibitors in patients with gastric adenocarcinoma

[0140] Based on the comparison of 91 patients with advanced gastrointestinal cancers treated with immune checkpoint inhibitors (PD-1 / PD-L1 inhibitors alone or in combination with CTLA-4 inhibitors) carrying germline HLA-B62 supertype, etc. Whether or not alleles are combined with the influence of tumor mutation burden on treatment outcome.

[0141] The specific experiment content is as follows:

[0142] 3.1 Experimental steps

[0143] 1) Collect tumor tissue samples and peripheral blood samples from 91 patients with advanced gastrointestinal cancer before immunotherapy. The peripheral blood samples are separated by two-step centrifugation to obtain blood cell samples. The fresh tumor tissue samples are crushed and processed into a cell suspension. Paraffin-embedded (FFPE) tissue samples were drawn into the ...

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Abstract

The invention provides a marker combination for predicting the treatment effect of a gastrointestinal tumor immune checkpoint inhibitor and application of the marker combination. The marker combination comprises at least one of HLA-B62 supertypes. According to the marker combination provided by the embodiment of the invention, the treatment effect of the gastrointestinal tumor immune checkpoint inhibitor of an object can be accurately predicted, for example, a PD-1 / PD-L1 inhibitor is singly used or combined with a CTLA-4 inhibitor, so that the response rate of treatment of the gastrointestinal tumor immune checkpoint inhibitor is improved.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular, to a combination of markers for predicting the curative effect of gastrointestinal tumor immune checkpoint inhibitors and its application, more specifically, to a combination of markers for predicting the curative effect of gastrointestinal tumor immune checkpoint inhibitors , the use of the combination of markers in predicting the curative effect of gastrointestinal tumor immune checkpoint inhibitors, a computer readable storage medium, an electronic device for predicting the curative effect of gastrointestinal tumor immune checkpoint inhibitors in a subject, and a method for predicting the curative effect of gastrointestinal tumor immune checkpoint inhibitors in a subject A method for the efficacy of tumor immune checkpoint inhibitors, a system for predicting the efficacy of gastrointestinal tumor immune checkpoint inhibitors in a subject, and a kit for predicting the efficacy of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G16B20/20
CPCC12Q1/6886G16B20/20C12Q2600/106C12Q2600/156
Inventor 张恒辉沈琳张磊鲁智豪杨滢李健孙怀博张小田杜波彭智
Owner GENE CRAB BIOTECH CO
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