Application of niacinamide in improving transparency of crystalline lens
A nicotinamide and lens technology, which is applied in the field of regenerative lens model construction and can solve problems such as improving the transparency of the regenerative lens
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Embodiment 1
[0032] Male C57BL / 6 mice (age, 5 weeks, body weight, 14-20 g) were purchased from Beijing Weitong Lihua Biological Co., Ltd. The mice were kept in a pathogen-free environment in the animal room of Shandong Ophthalmology Institute.
[0033]After the mice were killed by neck dislocation, the eyeballs were taken out and placed in a 1.5ml EP tube (PBS containing double antibodies) and placed in an ice box. After alcohol disinfection, the eyeballs were washed twice with PBS containing double antibodies again in the ultra-clean bench . Under a stereomicroscope, use ophthalmic scissors to cut the cornea along the limbus, separate the iris with the help of microscopic forceps, and break the zonule. Before removing the lens, use ophthalmic scissors to cut a small opening on the anterior capsule to mark the front capsule. When removing the lens, avoid surrounding cluttered tissue. After the lens was taken out, it was rinsed twice in PBS again to remove the surrounding tissue as much ...
Embodiment 2
[0038] The difference from Example 1 is that when preparing low-melting point agarose gel, the amount of low-melting point agarose powder is 0.15 g, and during cultivation, from day 1 to day 7, the culture medium is added bFGF on the basis of basal medium 5ng / ml; from the 8th day to the 14th day, the medium was added bFGF 200ng / ml on the basis of the basal medium, and when the culture was continued from the 15th to the 28th day, the medium was based on the basal medium Add bFGF 200ng / ml and nicotinamide 20μM. The basal medium is composed of DMEM / F-12, 1% B27, 1% L-glutamine, 1% double antibody, 5% FBS, and the rest are the same as in Example 1.
Embodiment 3
[0040] The difference from Example 1 is that when the culture is continued from the 15th day to the 28th day, the medium is supplemented with 200 ng / ml of bFGF and 13 μM of nicotinamide on the basis of the basal medium. All the other are the same as in Example 1.
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