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Recombinant baculovirus co-expressing porcine interferon L3 and porcine interleukin 22 and application

A technology for recombining baculovirus and porcine interferon, applied in the field of genetic engineering

Pending Publication Date: 2022-07-12
秦皇岛摩登狗生物科技有限公司 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the co-expression of porcine interferon L3 and porcine interleukin 22 using the baculovirus / insect cell expression system

Method used

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  • Recombinant baculovirus co-expressing porcine interferon L3 and porcine interleukin 22 and application
  • Recombinant baculovirus co-expressing porcine interferon L3 and porcine interleukin 22 and application
  • Recombinant baculovirus co-expressing porcine interferon L3 and porcine interleukin 22 and application

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Embodiment Construction

[0073] Various preferred embodiments of the present invention will be described below with reference to the accompanying drawings. The following description with reference to the accompanying drawings is provided to assist in an understanding of example embodiments of the present invention as defined by the claims and their equivalents. It includes various specific details to aid understanding, but they are to be regarded as exemplary only. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the invention. Also, detailed descriptions of functions and constructions that are well known in the art will be omitted for the purpose of making the description clearer and concise.

[0074] 1 Construction of baculovirus expression vector

[0075] Gene optimized synthesis of rPoIFN-L3 and rPoIL-22, rPoIFN-L3 contains FC tag, rPoIL-22 contains ...

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Abstract

The invention discloses recombinant baculovirus for co-expression of porcine interferon L3 and porcine interleukin 22 by using a baculovirus / insect cell expression system. Indirect immunofluorescence and Western-blot prove that the rPoIFN-L3 and the rPoIL-22, which are co-expressed, can be secreted and expressed in a cell supernatant. The co-expressed rPoIFN-L3 and rPoIL-22 have good biological activity, and can be used for effectively inhibiting the infection of TGEV / PEDV / PDCoV in vitro respectively. In addition, the research finds that the rPoIFN-L3 and the rPoIL-22 have a synergistic anti-TGEV / PEDV effect in vitro when the concentration is 50ng / mL. A large number of rPoIFN-L3 and rPoIL-22 with biological activity can be obtained through the protein expression system, and a foundation is laid for further realization of industrialized preparations for resisting porcine intestinal coronavirus infection.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the application of a baculovirus / insect cell expression system to co-express porcine interferon L3 and porcine interleukin 22 recombinant baculovirus and its application. Background technique [0002] Diarrhoeal diseases caused by enteroviruses are very common in the pig industry and have important economic significance, of which coronaviruses are the most important, which consists of four genera: alpha, beta, gamma and delta. Among the several porcine enteric coronaviruses that have been found, transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and the newly emerging swine acute diarrhea syndrome coronavirus (SADS-CoV) in 2017 belong to the alpha group. The coronavirus, porcine deltacoronavirus (PDCoV), is a deltacoronavirus. The survey results of swine diarrhea-related viruses in southern China from 2012 to 2018 showed that PEDV was the most f...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/866C07K14/54C07K14/555A61K38/20A61K38/21A61P31/14C12R1/93
CPCC12N7/00C12N15/86C07K14/54C07K14/555A61K38/20A61K38/21A61P31/14C12N2710/14021C12N2710/14043C12N2800/105A61K2300/00
Inventor 刘平黄徐发荣
Owner 秦皇岛摩登狗生物科技有限公司
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