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Method for evaluating toxicity of cosmetic basic raw materials to infant development by using zebra fish

A basic raw material, zebrafish technology, applied in the direction of analysis of materials, material inspection products, climate change adaptation, etc., can solve the problems of long experimental period, large animal damage, and complicated experiments, and achieve reliability, high accuracy, and experimental results. Simple operation effect

Pending Publication Date: 2022-08-05
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the evaluation indicators of these methods are highly subjective, rely on the experience of the implementer, and the experimental results are highly uncertain; at the same time, animal experiments are harmful to animals, do not conform to the 3R principle, and have problems such as long experimental periods and complicated experiments.

Method used

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  • Method for evaluating toxicity of cosmetic basic raw materials to infant development by using zebra fish
  • Method for evaluating toxicity of cosmetic basic raw materials to infant development by using zebra fish
  • Method for evaluating toxicity of cosmetic basic raw materials to infant development by using zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The selection of embodiment 1 basic raw material safety concentration to be tested

[0033] 1) Select the wild zebrafish AB strain embryos that have just been fertilized successfully, cultivate for 24-72h, and remove the dead embryos during the period;

[0034] 2) All remaining embryos are divided into five groups, wherein one group is the control group, and the other four groups are the treatment groups, each group has 20 embryos, and each group is set up with three repeat groups;

[0035] 3) select DMSO to dissolve phenoxyethanol, then prepare the medicinal liquid of 0, 5, 10, 20, 40 μg / mL with the culture medium, according to the size of the feeding vessel, prepare medicinal liquids of different volumes, if put into the culture dish of 6cm It is more suitable for the volume of the preparation liquid to exceed 5mL, and it is necessary to ensure that the DMSO concentration in each group is less than 1 / 1,000 to ensure that DMSO will not cause damage to the embryo;

[0...

Embodiment 2

[0039] Example 2 Detection of the effect of phenoxyethanol solution treatment on the heart rate of zebrafish embryos

[0040] 1) Select the wild zebrafish AB strain embryos that have just been fertilized successfully, cultivate for 24-72h, and remove the dead embryos during the period;

[0041] 2) All remaining embryos are divided into three groups, wherein one group is the control group, and the other two groups are the treatment groups, each group has 20 embryos, and each group is set up with three groups of repetition groups;

[0042]3) select DMSO to dissolve phenoxyethanol, and then prepare the medicinal liquid of 0, 5, and 10 μg / mL with culture medium, and wherein it is necessary to ensure that the DMSO concentration in each group is less than one thousandth to ensure that DMSO will not cause damage to the embryo;

[0043] 4) Transfer the zebrafish embryos to the prepared culture medium for exposure, and then place them in a constant temperature light-controlled incubato...

Embodiment 3

[0046] Example 3 Detection of the effect of phenoxyethanol solution treatment on the development of zebrafish

[0047] 1) Select the wild zebrafish AB strain embryos that have just been fertilized successfully, cultivate for 24-72h, and remove the dead embryos during the period;

[0048] 2) All remaining embryos are divided into three groups, wherein one group is the control group, and the other two groups are the treatment groups, each group has 20 embryos, and each group is set up with three groups of repetition groups;

[0049] 3) select DMSO to dissolve phenoxyethanol, and then prepare the medicinal liquid of 0, 5, and 10 μg / mL with nutrient solution, and wherein need to ensure that the DMSO concentration in each group is less than one thousandth;

[0050] 4) Transfer the zebrafish embryos to the prepared culture medium for exposure, and then place them in a constant temperature light-controlled incubator for cultivation, and observe once every 24 hours;

[0051] 5) After...

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Abstract

The invention provides a method for evaluating the toxicity of cosmetic base materials to infant development by using zebrafish, which comprises the following steps: (1) equally selecting zebrafish embryos which develop for 24-72 hours from fertilization, exposing the zebrafish embryos in a plurality of groups of embryo culture solutions containing base materials with different gradient concentrations, and culturing for 72 hours, selecting a basic raw material concentration which has a hatching rate of more than 95% and does not cause zebrafish body organ injury and development malformation as a safe concentration; (2) averagely dividing zebrafish embryos developed for 24-72 hours from fertilization into a control group and a plurality of treatment groups, exposing the zebrafish in the treatment groups in an embryo culture solution with a safe-concentration basic raw material for culturing, and after culturing for 72 hours, measuring and evaluating heart rate, development malformation degree, ROS oxidative stress and growth and development related gene expression conditions, so as to obtain the zebrafish embryos. And evaluating the developmental toxicity of the to-be-detected basic raw material. The method disclosed by the invention is simple and convenient to operate, beneficial to large-batch raw material detection and screening work, and relatively high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of cosmetic toxicity evaluation, and in particular relates to a method for evaluating the developmental toxicity of cosmetic basic raw materials to infants and young children by utilizing zebrafish. Background technique [0002] Cosmetics suitable for infants and young children have always been considered safe and reliable, but the specific safety needs to be determined. There are requirements for daily chemical products for infants and children in relevant daily chemical product standards and regulations, such as: "Cosmetics Safety Technical Specifications" (2015 Edition), "GB / T 29679-2013 Shampoo", "GB 8372- 2008 Toothpaste", "QB / T 1859-2013 Body Powder", "QB / T 1994-2013 Body Wash", etc., all have higher requirements on baby products than adult products. The market of daily chemical products for infants and children is getting wider and wider, and the number of purchasers and users is increasing year by y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/543
CPCG01N33/5014G01N33/543Y02A40/81
Inventor 张阳张璇付寅王紫藩蒋旭峰王卫国李亮韩新彬
Owner EAST CHINA UNIV OF SCI & TECH
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