Biomarker for detecting plateau hypoxia and application thereof

A technology of biomarkers and detection markers, applied in biological testing, material inspection products, etc., to achieve the effect of facilitating the progress of hypoxic injury and being easy to accept

Pending Publication Date: 2022-08-09
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of relatively mature, quantifiable, objective and sensitive clinical diagnostic indicators

Method used

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  • Biomarker for detecting plateau hypoxia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Animals simulate high altitude hypoxia exposure

[0019] 1. Select qualified experimental animals C57BL / 6 mice, male, purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd., raised in a constant temperature (23±2℃), constant humidity animal room, 12 / 12h day and night cycle every day, can be Free food and water. The hypobaric and hypoxic animal experimental cabin simulating the plateau environment was purchased from Guizhou Fenglei Aviation Ordnance Co., Ltd., model: DYC-DWI. The cabin is connected with two air pumps, which can continuously pump the gas in the cabin out of the cabin, thereby simulating a low-pressure and low-oxygen environment at a certain altitude; at the same time, an electronic display screen and an oxygen sensor probe are connected (real-time display of the location in the cabin). altitude and oxygen concentration).

[0020] 2. 18 mice were randomly divided into three groups, control group, hypobaric hypoxia group for...

Embodiment 2

[0022] Embodiment 2 IL-5 content detection

[0023] The detection method of IL-5 in serum is carried out according to the following steps:

[0024] (1) Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20 minutes, and seal the remaining slats in a self-sealing bag and put them back at 4°C;

[0025] (2) Set standard wells (concentrations are 0, 5, 10, 20, 40, 80 pg / ml in sequence) and sample wells, and add 50 μl of standard with different concentrations to each standard well;

[0026] (3) First add 10 μl of the sample to be tested to the sample well, and then add 40 μl of the sample diluent; the blank well is not added;

[0027] (4) In addition to the blank wells, add 100 μl of horseradish peroxidase (HRP)-labeled detection antibody to each well of the standard wells and sample wells, seal the reaction wells with a sealing film, and incubate at 37°C for 60 minutes;

[0028] (5) Discard the liquid, pat dry with absorbent paper, ...

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Abstract

The invention discloses a biomarker for detecting plateau hypoxia injury and application of the biomarker. The biomarker is interleukin 5 or a specific antibody thereof, and is used for preparing a detection reagent or kit for plateau hypoxia. The biomarker provided by the invention can be used for serum marker detection to judge the plateau hypoxia degree of an organism, and is beneficial to detection or auxiliary detection of the hypoxia state, so that corresponding treatment measures can be taken as early as possible. The marker is convenient and rapid to detect, can be easily accepted by patients, and is more convenient for dynamically monitoring the progress of hypoxia injury of plateau people.

Description

technical field [0001] The invention belongs to the technical field of detecting plateau hypoxia, in particular to a biomarker for detecting plateau hypoxia and its application. Background technique [0002] The plateau has a special natural environment, which is characterized by low pressure, low oxygen, dry and cold climate, high wind speed, and significantly increased solar radiation and ultraviolet radiation. In the plateau environment, with the increase of altitude, the partial pressure of oxygen in the air continues to decrease. If people stay in this hypoxic environment for a long time, severe hypoxemia may occur. Because human nerve tissue is most sensitive to changes in the internal and external environment, under hypoxic conditions, brain damage occurs at the earliest, and the degree of damage is more serious, and the longer the exposure time, the more serious the damage, especially for sensation, memory, thinking The effects on cognitive functions such as attenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6869G01N2333/5409G01N2800/7038
Inventor 朱玲玲赵名成祥史子毕
Owner ACADEMY OF MILITARY MEDICAL SCI
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