Extraction of milk vetch polysaccharide by enzyme treatment

A technology of astragalus polysaccharide and enzyme treatment, which is applied in the direction of fermentation, can solve the problems of extracting astragalus polysaccharide that have not been seen, and achieve the effect of mild conditions, maintaining activity and accelerating disintegration

Inactive Publication Date: 2006-07-05
CHINA AGRI UNIV
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Problems solved by technology

However, there is no report on the extraction of astragalus polysaccharides by enzyme treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A Degreasing: Add 1 kg of astragalus powder to 6 liters of ethanol, reflux for extraction for 1.5 hours, and collect the filter residue by filtration.

[0029] B enzyme treatment: above-mentioned filter residue is evaporated dry solvent for subsequent use, adds 2g hemicellulase (made by Streptomyces cirratus (self-made by Streptomyces cirratus), preparation method sees below) in 8 liters of water, adjusts pH value to 5.5, and Filter residue and mix well. Keep warm at 50-60°C and stir for 2 hours.

[0030] C Heat the above mixture to a slight boil, extract for 2 hours, filter, and the filtrate is the polysaccharide extract A.

[0031] The filter residue is ready for use.

[0032] D Add 6 liters of water to the filter residue obtained in step C, extract by microboiling for 1 hour, filter, and the filtrate is polysaccharide extract

[0033] Take solution B.

[0034] E Combine the polysaccharide extracts A and B obtained in step C and step D, concentrate under reduced p...

Embodiment 2

[0044] A Degreasing: Add 1Kg of astragalus powder to 4 liters of ethanol, reflux and extract for 1 hour, and filter to collect the filter residue.

[0045] B Enzyme treatment: evaporate the above filter residue to dry the solvent for later use, add 2 g of papain (Papain enzymatic code EC 3.4.22.2) to 8 liters of water, adjust the pH value to 6.7, and mix with the filter residue. Keep warm at 60-70°C and stir for 1 hour.

[0046] C Heat the above mixture to a slight boil, extract for 1.5 hours, filter, and the filtrate is the polysaccharide extract A. The filter residue is ready for use.

[0047] D add 4 liters of water to the filter residue obtained in step C, add commercially available cellulase (from Aspergillus niger) and hemicellulase (prepared by Streptomyces-streptomyces cirratus, method is the same as in Example 1) each 0.5g, Adjust the pH value to 5.5, keep warm at 50-60°C, and stir for 1 hour. Extract by slight boiling for 1 hour, filter, and the filtrate is polysa...

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PUM

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Abstract

An enzyme treating method for extracting astragalus polyose includes such steps as defatting, enzyme treating, extracting, depositing in alcohol, drying and pulverizing. The used enzyme is chosen from cellulose, semi-collulase, proteinase, pectinase and beta-amylase. Its advantages are high output rate and high purity.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides in Astragalus membranaceus by using enzyme treatment. Background technique: [0002] Radix Astragali (Radix Astragali) is the dried root of Astragalus membranaceus (Fisch) Bge.Var.mongholicus (Bge) Hsiao. or A. membranaceus (Fisch) Bge. of the leguminous plant Astragalus membranaceus (Fisch) Bge. Astragalus polysaccharide is one of the main components of Astragalus, which has a wide range of pharmacological effects. [0003] The traditional method of extracting astragalus polysaccharides is mainly direct water extraction or water extraction after degreasing. The medicinal part of astragalus is the root, in which there are more cellulose, hemicellulose and lignin, and the release of active ingredients during the extraction process has a greater impact. hindering effect, so the extraction rate is low. Although newer extraction methods such as ultrasonic or microwave methods have certain e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04
Inventor 阎巧娟韩鲁佳江正强刘向阳刘依
Owner CHINA AGRI UNIV
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