Spodoptera exigua Multi-nucleocapside Nucleopolyedrovirus and its bioinsecticide

The technology of nuclear polyhedrosis virus and biological insecticide is applied in the field of beet armyworm nuclear polyhedrosis virus and its biological insecticide, which can solve the problems of increasing the difficulty of beet armyworm and achieve the effect of strong specificity

Inactive Publication Date: 2002-05-01
武汉普洛森农产品供应链有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, beet armyworm has a strong migratory habit, which greatly increases the difficulty of traditional methods to control beet armyworm

Method used

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  • Spodoptera exigua Multi-nucleocapside Nucleopolyedrovirus and its bioinsecticide
  • Spodoptera exigua Multi-nucleocapside Nucleopolyedrovirus and its bioinsecticide

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The viruses of the present invention and their bioinsecticides are described in further detail below.

[0014] Isolation and Amplification

[0015] The virus of the present invention was isolated from a virus-killed beet armyworm larvae found in a place in Anyang, Henan in 2000. The collected insect carcasses were mashed and dropped on the surface of the feed to feed the beet armyworm larvae to artificially multiply the virus. The infection was carried out at 27°C, and the above process was repeated to amplify a large amount of beet armyworm virus, which was stored in a 4°C refrigerator.

[0016] purification

[0017] The virus was propagated in Spodoptera exigua larvae according to the above method, and the infection symptoms of the larvae were consistent with the typical virus infection symptoms: the body knot was swollen and shiny, the body color became pale, the epidermis was thin and brittle, and it was broken when touched, and a milky white thick liquid was exud...

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Abstract

The present invention relates to a Spodoptera exigue Multi-nucleocapside Nucleopolyhedrovirus (SeMNPV), whose storage number is V200107, its biologic insecticide and the application of said insecticide in controlling the pests of vegetable, fruit and agricultural crops, such as spodoptera exigua multi-nucleo capside, autographa califorica, etc. Its advantages are high specificity, no generation of resistance and no environmental pollution.

Description

technical field [0001] The present invention relates to a beet armyworm nuclear polyhedrovirus (Spodoptera exigua Multi-nucleocapside Nucleopolyhedrovirus, SeMNPV) and a biological insecticide thereof. Insecticides and their use in pest control. The virus strain has been deposited in the China Center for Type Culture Collection on September 7, 2001, which is abbreviated as CCTCC, and the deposit number is CCTCC No.V200107. Background technique [0002] Beet armyworm (Spodoptera exigua Hbner) belongs to Lepidoptera, Noctuidae, Amphypyninae, Spotera Guenee, and is a worldwide distributed Crop pests have serious damage records in Asia, America, and Europe. [0003] Beet armyworm is a tropical pest, but it can also reproduce at low temperature. To herbs, from crops to medicinal materials can be harmful. Among the crops, beans, peanuts, vegetables, corn, and cotton are the most serious hazards. [0004] The damage of Spodoptera littoralis has the following characteristics: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N7/01
Inventor 张俊杰未荣杰张友清
Owner 武汉普洛森农产品供应链有限公司
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