Establishment of C578L/6 mouse animal model of transbuman senile dementia APP gene and use

A technology of APP gene and animal model, applied in preparations for in vivo experiments, pharmaceutical formulas, etc., can solve the problem of lack of AD animal models

Inactive Publication Date: 2005-10-26
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

[0004] Although the research on AD in my country has made some progress, there is still a lack of suitable animal models of AD, which has become a bottleneck problem that limits the research of drug screening, vaccine evaluation and pathogenesis for the prevention and / or treatment of AD.

Method used

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Embodiment Construction

[0028] The present invention will further illustrate by embodiment:

[0029] 1. Using transgenic technology, use PDGF promoter to regulate APP cDNA 695, 751V 717 to construct transgene.

[0030] 2. The transgene fragment was injected into the male pronucleus of the fertilized egg of the mouse by microinjection.

[0031] 3. Use PCR and Scuthern hybridization to detect the positive mice obtained, and determine the first positive mouse C 57 BL / 6 mice.

[0032] 4. The first positive mice were mated with siblings,

[0033] 5. Through PCR and Southern identification, transgenic positive animals were obtained.

[0034] 6. Transgenic positive animals were delivered by cesarean section for microbial purification.

[0035] 7. Animals positive for microbial transgenes were mated with compatriots of the same ancestry for 10 generations.

[0036] 8. Establish homozygous homologous recombination inbred lines.

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Abstract

A mouse animal model C57BL / 6 for the APP gene of human presenile dementia (AD) is disclosed, which can be used to research the AD, develop its medicine and screen its vaccine. Its configuring process includes using PDGF promoter to regulate APP and configure its transgene, injecting it in the male pronucleus of fertilized ovum of mouse, PCR, southern discrimination to obtain transgenic male animal, cesarean section, and DNA recombination-inbred-line passage for configuring its line.

Description

technical field [0001] The present invention relates to a kind of animal model of APP gene of transhuman senile dementia, especially C 57 BL / 6 mouse animal model, its construction method and application. It is mainly used in the fields of life sciences and pharmaceutical research and development. Background technique [0002] With the increase of average human life expectancy, human society has entered into an aging society. With the aging of the population, Alzheimer's disease (AD) has become the fourth leading cause of death in adults after heart disease, cancer, and AIDS, and more than 100,000 people die from AD each year. The incidence rate in my country is close to 10%, and it is estimated that there are no less than 20 million AD patients in China. At present, there is no western medicine that can effectively treat Alzheimer's disease. Traditional Chinese medicine has become one of the hopes for the treatment of senile dementia, and it is also a field of research a...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 秦川张连峰朱华高虹黄澜
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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