Method for rapidly detecting rice seed purity during seedling stage

A rice seed and seedling stage technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of time lag in obtaining results, long identification time, labor-intensive and other problems, and achieve the effect of accurate identification

Inactive Publication Date: 2005-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the identification time is too long to meet the verification needs of the seed trade. It is labor-intensive and time-consuming, and the time to obtain the results is mostly lagging behind the seed sales behavior.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Convenient detection of seed purity at the seedling stage of three-line hybrid rice: The CMS line A-ZS1 and the restorer line Minghui 63 were selected in the seedling growth stage of the three-line hybrid rice cytoplasmic male sterile line Zhenshan 97A Take the preparation of the first generation of hybrids or the purity test of A-ZS1 male sterile line breeding seeds as an example. Sampling and marking the first generation of hybrids prepared by the rice CMS or the propagated CMS A-ZS1 seeds are germinated indoors at room temperature (about 25° C.) for 1-2 weeks or outdoor sowing for 1-2 weeks. For the first-generation hybrid seeds, if they are normal green seedlings, they are true hybrids of A-ZS1 and Minghui 63; The seed purity of the first generation of hybrid between A-ZS1 and Minghui 63; for the seed of CMS A-ZS1, if it is a normal green seedling, it is a false seed of string powder; if it is a marked seedling, it is a true CMS A-ZS1, and This method intuitively a...

Embodiment 2

[0015] Convenient detection of seed purity in the seedling stage of two-line hybrid rice: the male sterile line A-Y1 and the restorer line 838 were used to select the two-line hybrid rice photothermosensitive male sterile line Y22S to prepare the first generation of hybrids or carry out The purity test of A-Y1 male sterile line breeding seeds is taken as an example. Sampling the first generation hybrid prepared by marking the rice sterile line or the propagated sterile line A-Y1 seeds, germinated indoors at room temperature (about 25° C.) for 1-2 weeks or sowed outdoors for 1-2 weeks. For the first-generation hybrid seeds, if they are normal green seedlings, they are true hybrids of A-Y1 and 838; The seed purity of the first generation of hybrids between Y1 and 838; for the seeds of the sterile line A-Y1, if it is a normal green seedling, it is a false seed of string powder, and if it is a marked seedling, it is a true sterile line A-Y1, which is intuitive and fast Calculate ...

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Abstract

The method for quickly and conveniently detecting rice seed purity in seedling stage includes the following steps: utilizing hybrid generation prepared by hybrid rice sterile line whose character is expressed and labeled by seedling growth specifity or reproductive sterile line seed, making indoor germination or outdoor sowing test; for hybrid generation calculating the ratio of labeled seedlings mixed in seedlings with normal green color to define seed purity of hybrid generation, and for sterile line calculating the ratio of normal green seedlings mixed in labeled seedlings to define seed purity of sterile line.

Description

technical field [0001] The invention relates to a method for conveniently detecting the purity of rice seeds at the seedling stage. Background technique [0002] The identification methods of hybrid rice hybrid purity currently in my country mainly include morphological character identification, isozyme and protein electrophoresis identification, and molecular marker identification. The isozyme and protein electrophoresis identification methods can detect relatively few sites, and the ability to identify closely related varieties is poor, and the expression of isozymes and proteins is easily affected by environmental conditions. Variety identification by molecular marker identification is a very promising research direction. However, these two identification methods require certain experimental skills and operating experience, and are equipped with necessary equipment and biochemical reagents. It is difficult to popularize the technology and most of them are still in the ex...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H1/02
Inventor吴殿星舒庆尧
OwnerZHEJIANG UNIV