Expression method of glucosal related protein and application thereof

Abstract

The express and adjust method for GRP in bio-medicine field comprises: modifying the GRP into secretory protein to load into the tumor cell cultured externally; then, the altumen stimulates the body anti-tumor immunity function to prepare tumor-vaccine and cure tumor; while injecting directly the albumen adenovirus to infect the tumor cell and express the albumen for tumor treatment with chemotherapy and radiotherapy.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for expressing a sugar-regulating protein, in particular to a method for expressing a sugar-regulating protein in tumors and its application. Background technique [0002] Tumor cells contain a large number of gene mutations, about 11,000 mutations / cell, so each tumor has its own unique antigen peptide, which has the potential to cause tumor immune response. However, in general, tumor cells cannot elicit a sufficient immune response, and one of the most important reasons is that the function of antigen-presenting cells is insufficient or unwell. Therefore, enhancing the presentation of tumor antigens may elicit effective anti-tumor immune responses. [0003] Members of the heat shock protein (Heat Shock Protein, HSP) family, such as HSP 70 and HSP 90, have the function of molecular chaperon (chaperon), assisting newly synthesized proteins to complete the secondary and tertiary s...

AI Technical Summary

Problems solved by technology

However, the extraction of HSP from tumor tissue has great limitations in practical application.

Such as the loss of tumor antigen peptides during the purification process, the amount of HSP extraction is limited by the size of the tumor

Moreover, extracting HSP for each tumor is expensive, and it is difficult to establish a standardized protocol, which is difficult in clinical application.

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