Inhibitors of phosphodiesterases in infertility
a technology of phosphodiesterases and infertility, which is applied in the field of inhibitors of phosphodiesterases in infertility, can solve the problems of producing ovarian hyperstimulation syndrome (ohss), affecting the development of ovarian follicles, and affecting the development of ovaries, so as to stimulate follicle growth and maturation, stimulate follicle growth and increase follicle maturation, the effect o
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
Ovulation Induction Using Various PDE Inhibitors in Combination with Low-Dose FSH
[0141] Experiments were conducted according to the In Vivo Rat Follicle Maturation Assay, described above. In one experiment, ovaries were harvested from rats at midday of the second day of treatment for histological analysis, prior to the last set of injections. These rats received only the first three doses of FSH and test compound prior to euthanasia and organ harvest. The secondary follicles were evaluated by counting the total number of secondary follicles: including both small follicles (those at any intermediate stage of maturation, having a multilayered granulosa with the first, scattered vacuoles, but without an antrum) and antral follicles (those having antral dilation, with an external diameter of around 500 microns, or higher, with or without thinning of the granulosa cell layer). The antral follicles (≧500 mcm) were also counted separately.
[0142] The proportion of ovulatin...
example 2
Induction of cAMP Using Various PDE Inhibitors in Combination with Low-Dose FSH
[0165] In addition to the evaluation of rat granulosa cells, produced as described above, in order to measure cAMP in cell lines, JC-410 porcine granulosa cells were used. These cells were obtained from Dr. Jorge Chedrese (University of Saskatchewan). The cells were maintained in DMEM / F12 supplemented with 5% newborn calf serum (Gibco) and 5 μg / ml of insulin (Gibco). Stable cell lines were established by transfecting the cDNAs for the human LH and FSH receptors into the cells using standard transfection techniques and selection with 300 μg / ml of Geneticin (Gibco). Following selection, the cells were maintained in the same concentration of Geneticin. For cAMP determinations, the cells were plated at a density of 25,000 cells per well, in 96 well plates, one day prior to the assay. The following day, the cells were stimulated for 1 hour with increasing doses of the inhibitor molecules in the presence, or a...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com