Methods and Compositions for Preventing a Condition
a technology of compositions and methods, applied in the field of methods and compositions for preventing a condition, can solve the problems of few effective vaccines available, reduce one or more symptoms, and prevent or reduce the likelihood of the subject developing cancer
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example 1
[0274]C57BL / 6 mice were immunized with 2 μg of the constructs (FIG. 1), and were delivered as single injection in 100 μl of PBS or PBS formulated with Vaxfectin. Mice received three immunizations at bi-weekly intervals (i.e. over 6 weeks). For the control group, 105 (initial immunization) and 5×104 (booster immunizations) irradiated P. yoelii sporozoites (17XN) were inoculated by tail-vein injection at the same time-points. All challenges were accomplished by injecting 5×103 sporozoites in the tail vein two weeks after last immunization. Results for antibody responses are illustrated in FIG. 2; results for protective efficacy against sporozoites challenge are illustrated in FIG. 3; and results for antibody neutralization activity are illustrated in FIG. 4.
example 2
[0275]C57BL / 6 mice were immunized with 2 μg of pCSP or pMCSP constructs (see FIG. 1) and were delivered as single injection in 100 μl of PBS formulated with Vaxfectin. Mice received three immunizations at bi-weekly intervals (i.e. over 6 weeks). To deplete the CD4+, CD8+, or both T cell subsets, immunized mice were injected (intraperitoneal; i.p) with anti-CD4, anti-CD8, or both mAbs two weeks after last immunization. Twenty four hours later, the efficacy of the depletion was estimated by two-color flow cytometry analysis of peripheral blood lymphocytes using FITC-conjugated anti-CD4 or APC-conjugated anti-CD8 mAbs (FIG. 5). Data show the CD4 and CD8 expression on combined peripheral lymphocytes of three mice in each group. Sporozoites challenge was performed by injecting 2500 sporozoites in mice tail vein. FIG. 6 illustrates protection mediated by immunization with Vaxfectin-formulated CSP or MCSP. Antibody response is illustrated in FIG. 7 and antibody neutralization activity is i...
example 3
[0276]C57BL / 6 mice were immunized with 100 μl PBS or 2 μg of Vaxfectin formulated plasmids DNA (100 μl) (see FIG. 1). 24 or 48 hours later, injected muscles are harvested and total RNA is isolated. Indicated cytokine or chemokine levels were analyzed by real-time PCR.
[0277]FIG. 9 illustrates real-time PCR evaluation of expression levels of cytokines (24 h after immunization). FIG. 10 illustrates real-time PCR evaluation of expression levels of cytokines (48 h after immunization).
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