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Flow cytometery system with fluidics control system

a flow cytometer and control system technology, applied in the field of flow cytometery systems, can solve the problems of unreliability of extra control devices and overcomplicated fluid control systems

Inactive Publication Date: 2019-09-19
CYTEK BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a system, method, and device for flow cytometry fluidics in a flow cytometer. The system uses dual laser devices and scatter channels to measure the velocity of particles in a sample fluid. The device also includes a stepper motor modulated valve to control the flow of sample and sheath fluids, which affects the particle velocity in the flow channel. Overall, this system improves the accuracy and reliability of flow cytometry analysis.

Problems solved by technology

Moreover, an overcomplicated fluid control system may be unreliable with extra control devices.

Method used

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Embodiment Construction

[0031]In the following detailed description of the embodiments, numerous specific details are set forth. However, it will be obvious to one skilled in the art that the embodiments may be practiced without these specific details. In other instances well known methods, procedures, components, and circuits have not been described in detail so as not to unnecessarily obscure aspects of the embodiments.

[0032]A system, method, and apparatus are provided for flow cytometry fluidics. The system includes dual laser devices and dual scatter channels to measure time differences and calculate velocity of particles in a core stream of sample fluid. The system also includes a stepper motor modulated flow control valve to control the proportion of sheath flow rate and sample flow rate in a flow channel. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on different...

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Abstract

A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This patent application is a continuation and claims the benefit of U.S. patent application Ser. No. 15 / 817,237 titled FLOW CYTOMETRY SYSTEM WITH FLUIDICS CONTROL SYSTEM filed on Nov. 19, 2017 by inventors David Vrane et al. U.S. patent application Ser. No. 15 / 817,237 claims priority to U.S. Provisional Patent Application No. 62 / 424,464 titled FLOW CYTOMETRY SYSTEM AND STEPPER MOTOR PINCH VALVE THEREFOR filed on Nov. 19, 2016 by inventors David Vrane et al.FIELD[0002]The embodiments of the invention relate generally to flow cytometry systems.BACKGROUND[0003]Flow cytometry involves the optical measurement of cells or particles of a test sample carried in a fluid flow. The collective instrumentation that achieves this task is known as a flow cytometer.[0004]The control of the flow fluid with the test sample in a flow cytometer is important to accurately analyze the type and quantity of cells or particles in the test sample. If the velocity o...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N15/14G01N21/49
CPCG01N15/1404G01N21/49G01N15/147G01N2015/1006G01N2015/1409G01N2015/1438G01N15/1434G01N15/1436G01N2015/1075G01N2015/1027G01N15/1409
Inventor VRANE, DAVID
Owner CYTEK BIOSCI
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