Method for inducing formation of coral fungus entity

Abstract

The invention relates to a method for inducing formation of a coral fungus entity. According to the technical scheme, the method comprises the steps that after coral fungus slope strains are activated, fungus blocks are taken to be inoculated into a PDA culture medium, constant temperature culture is carried out for 15-40 d at 25 DEG C to 28 DEG C, mycelia and a solid culture medium are cut into small blocks and are soaked into organic solvents, organic leach liquor is concentrated to obtain extracts, extraction is carried out through ethyl acetate and pure water with the ratio of 1:1, an ethyl acetate phase is dehydrated and evaporated to be dried, and coral fungus organic crude extracts are manufactured. The organic extracts are directly added to the coral fungus culture medium, or a certain number of components after further separation are added to the coral fungus culture medium, the formation of the coral fungus entity can be induced, growth is 7-10 days earlier than normal growth, and growth is even.

Description

technical field

[0001] The invention relates to a method for inducing the formation of fruit bodies of coral fungi, and relates to the field of artificial cultivation of edible and medicinal large fungi. Background technique

[0002] Fruiting body development is an important stage in the life history of macrofungi. After the vegetative growth of macrofungal mycelium, the mycelium is stimulated by environmental factors such as light and temperature for a certain period of time. The yield of fruiting bodies. There are 2000-5000 species of large-scale fungi in nature. There are about 1500-2000 species in my country, and 938 species have been reported, belonging to 166 genera. At present, less than 100 species can be cultivated artificially. 20 kinds. Although a large number of wild edible and medicinal fungi (such as coral fungus, cordyceps, russula, morel, boletus, etc.) have special medicinal value and rich nutritional value, it is difficult to obtain fruiting bodies through...

Examples

Embodiment 1

[0014] The induction steps of coral fungus fruiting body formation are as follows:

[0015] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0016] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a Petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 26°C for 25 days.

[0017] 3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract (ethyl acetate: methanol...

Embodiment 2

[0020] The induction steps of coral fungus fruiting body formation are as follows:

[0021] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0022] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 28°C for 15 days.

[0023] 3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract solution (ethyl acetate:...

Embodiment 3

[0026] The induction steps of coral fungus fruiting body formation are as follows:

[0027] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0028] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a Petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 25°C for 40 days.

[0029]3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract (ethyl acetate: methanol:...

technical field

[0001] The invention relates to a method for inducing the formation of fruit bodies of coral fungi, and relates to the field of artificial cultivation of edible and medicinal large fungi. Background technique

[0002] Fruiting body development is an important stage in the life history of macrofungi. After the vegetative growth of macrofungal mycelium, the mycelium is stimulated by environmental factors such as light and temperature for a certain period of time. The yield of fruiting bodies. There are 2000-5000 species of large-scale fungi in nature. There are about 1500-2000 species in my country, and 938 species have been reported, belonging to 166 genera. At present, less than 100 species can be cultivated artificially. 20 kinds. Although a large number of wild edible and medicinal fungi (such as coral fungus, cordyceps, russula, morel, boletus, etc.) have special medicinal value and rich nutritional value, it is difficult to obtain fruiting bodies through...

Examples

Embodiment 1

[0014] The induction steps of coral fungus fruiting body formation are as follows:

[0015] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0016] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a Petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 26°C for 25 days.

[0017] 3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract (ethyl acetate: methanol...

Embodiment 2

[0020] The induction steps of coral fungus fruiting body formation are as follows:

[0021] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0022] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 28°C for 15 days.

[0023] 3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract solution (ethyl acetate:...

Embodiment 3

[0026] The induction steps of coral fungus fruiting body formation are as follows:

[0027] 1) Prepare solid medium. The solid medium is potato dextrose medium (recipe: 200 g of peeled potatoes boiled for infusion, 20 g of glucose, 10 g of agar, 1 L of water), sterilized by high pressure steam at 121 °C for 30 min.

[0028] 2) Solid culture of coral bacteria. Coral fungus ( Clavicoronapyxidata , Clavicorolides A and B, sesquiterpenoids from the Fermentation Products of Clavicoronapyxidata. Organic Letters, 2009,11(1):109-112.) The slant strains are activated, and the activated slant strains are picked out by 0.5-1.0cm 2 The bacterial block was inoculated into a Petri dish (9 cm in diameter) containing 20 ml of the solid medium, and cultured at 25°C for 40 days.

[0029]3) Preparation of organic extracts of coralline bacteria. Cut the cultured coral fungus mycelia together with the solid medium into small pieces, soak them in the organic extract (ethyl acetate: methanol:...

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