Method for breaking psammophyte Agriophyllum squarrosum seed dormancy and improving germination rate

Abstract

The present invention discloses a method for breaking psammophyte Agriophyllum squarrosum seed dormancy and improving germination rate. The method comprises: selecting annual robust, full, undamaged and pest-free Agriophyllum squarrosum seed; soaking the Agriophyllum squarrosum seed for 6-12 h with water with a temperature of 30-40 DEG C under a dark condition, taking out, and carrying out air-drying; carrying out disinfection sterilization for 5-10 min with a 5% sodium hypochlorite solution, rinsing with distilled water, and carrying out air-drying; preparing 0.1-0.2 g / ml of gibberellin GA3, soaking the disinfected Agriophyllum squarrosum seed in the step 3) for 24 h with the gibberellin GA3 under a full dark condition; and placing the soaked Agriophyllum squarrosum seed into a culture dish spread with two layers of filtration paper, adding distilled water to moisten the filtration paper, and placing in a 25 DEG C full-dark constant temperature oven to culture to achieve the germination state. Compared with the method in the prior art, the method of the present invention has the following characteristics that the deep dormancy of the seed is broken, the Agriophyllum squarrosum seed germination rate is substantially increased, the Agriophyllum squarrosum seed germination period is shortened, and the seed germination rate is up to more than 90%.

Description

Technical field

[0001] The invention relates to the technical field of plant cultivation, in particular to a method for breaking the dormancy of the seeds of the sand plant Shami and improving the germination rate. Background technique

[0002] Agriophyllum squarrosum (Agriophyllum squarrosum) is an annual herbaceous plant in the Chenopodiaceae. It grows on mobile sand and semi-fixed sand. It is a typical sand plant and pioneer plant for sand fixation. Shami seeds are also a natural functional food. It is rich in essential mineral elements for the human body, which can help promote digestion and strengthen the spleen and stomach. The only breeding method of sand rice is seed reproduction. It reproduces in the arid and high temperature sand dunes. The natural germination rate is low. The reason is closely related to the harsh habitat. The deep dormancy of sand rice seeds is also an important factor for its low germination rate. Therefore, in order to realize artificial cultivatio...

Examples

Embodiment 1

[0020] 1) Select the annual healthy and full, non-damaged and pest-free Shami seeds in the Minqin area, and wash them for later use;

[0021] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0022] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0023] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0024] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is r...

Embodiment 2

[0026] 1) Select the annual healthy and full, non-damaged and pest-free Shami seeds in the Naiman area and wash them for later use;

[0027] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0028] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0029] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0030] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is re...

Embodiment 3

[0032] 1) Select the annual healthy, full, undamaged and pest-free Shami seeds in the Shapotou area and wash them for later use;

[0033] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0034] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0035] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0036] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is regar...

Technical field

[0001] The invention relates to the technical field of plant cultivation, in particular to a method for breaking the dormancy of the seeds of the sand plant Shami and improving the germination rate. Background technique

[0002] Agriophyllum squarrosum (Agriophyllum squarrosum) is an annual herbaceous plant in the Chenopodiaceae. It grows on mobile sand and semi-fixed sand. It is a typical sand plant and pioneer plant for sand fixation. Shami seeds are also a natural functional food. It is rich in essential mineral elements for the human body, which can help promote digestion and strengthen the spleen and stomach. The only breeding method of sand rice is seed reproduction. It reproduces in the arid and high temperature sand dunes. The natural germination rate is low. The reason is closely related to the harsh habitat. The deep dormancy of sand rice seeds is also an important factor for its low germination rate. Therefore, in order to realize artificial cultivatio...

Examples

Embodiment 1

[0020] 1) Select the annual healthy and full, non-damaged and pest-free Shami seeds in the Minqin area, and wash them for later use;

[0021] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0022] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0023] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0024] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is r...

Embodiment 2

[0026] 1) Select the annual healthy and full, non-damaged and pest-free Shami seeds in the Naiman area and wash them for later use;

[0027] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0028] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0029] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0030] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is re...

Embodiment 3

[0032] 1) Select the annual healthy, full, undamaged and pest-free Shami seeds in the Shapotou area and wash them for later use;

[0033] 2) Soak the Shami seeds in water at 30℃ for 6 hours in the dark, and then remove them to dry;

[0034] 3) Sterilize with 5% sodium hypochlorite solution for 10 minutes, then rinse with distilled water and dry;

[0035] 4) Gibberellin GA with concentrations of 0.0125g / ml, 0.025g / ml, 0.05g / ml, 0.075g / ml, 0.1g / ml, 0.15g / ml, 0.2g / ml 3 , Use the above-mentioned concentration of gibberellin GA to the sterilized Shami seeds in step 3) 3 Soak the seeds for 24h under the complete darkness of 25℃;

[0036] 5) Every 30 seeds of Shami seeds after soaking are placed in a petri dish covered with 2 layers of filter paper, and distillation is added to keep the filter paper moist, and placed in a full-dark incubator at 25°C until germination. The germination rate is counted every day. If the number of germination remains unchanged for 5 consecutive days, it is regar...

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