Flavonoid compound of Bupleurum marginatum and preparation method thereof

Abstract

The invention specifically relates to a novel flavonoid compound extracted and separated from Bupleurum marginatum and a preparation method thereof, belonging to the technical field of medicine. The compound has a chemical structure formula as shown in a formula I which is described in the specification. The preparation method for the flavonoid compound extracted and separated from Bupleurum marginatum comprises the following successive steps: extracting; concentration; extraction; silica gel column chromatographic separation; and Sephadex LH-20 chromatographic separation. The invention has the advantage that the extraction and separation method for the novel flavonoid compound extracted from Bupleurum marginatum is simple and has good reproducibility.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a novel flavonoid compound extracted and separated from Bupleurum bupleuri and a preparation method thereof. Background technique

[0002] Bupleurum marginatum Wall ex DC. is a plant of the genus Bupleurum in the family Umbelliferae. It has the functions of dispelling wind and reducing fever, soothing the liver and promoting yang. tone, uterine prolapse, anal prolapse, etc. Bamboo leaf Bupleurum is mainly distributed in Sichuan, Hubei, Yunnan, Guizhou and other provinces of China, and it is a local customary product of Bupleurum medicinal materials in southwest my country.

[0003] There are various types of chemical components in Bupleurum bupleuri, mainly containing saponins, flavonoids, lignans, coumarins, and volatile oils. Flavonoids are an important indicator of its quality control. The flavonoids involved in the present invention have no related patents or ...

Examples

Embodiment 1

[0027] Example 1 Preparation of 3,3',5'-trimethoxy-myricetin-7-O-β-D-glucoside from Bupleurum bupleuri

[0028] (1) 15 kg of Bupleurum bupleurum, adding 10 times of volume fraction of 75% ethanol and extracting under reflux for 3 times, each time for 1.5 hours, filtering, merging the extracts, concentrating and recovering the extracts under reduced pressure at 50°C, to obtain bamboo 0.92kg of ethanol crude extract of Bupleurum chinensis;

[0029] (2) Take the ethanol crude extract of Bupleurum bupleuri obtained in step (1) and suspend it in water, and use sherwood oil, ethyl acetate, n-butanol and other volume fractional extractions successively to prepare different polar parts, wherein n-butanol Alcohol part 250g;

[0030] (3) Mix the n-butanol part obtained in step (2) with 200-300 mesh silica gel according to the n-butanol part and silica gel mass ratio of 1:2, after drying, separate through 200-300 mesh silica gel column chromatography, With dichloromethane-methanol mixe...

Embodiment 2

[0034] Example 2 Preparation of 3,3',5'-trimethoxy-myricetin-7-O-β-D-glucoside from Bupleurum bupleuri

[0035] (1) 200 kg of Bamboo Leaf Bupleurum Radix, adding 10 times of volume fraction of 75% ethanol and extracting under reflux for 2 times, each time for 2 hours, filtering, merging the extracts, concentrating and recovering the extracts under reduced pressure at 55° C. to obtain bamboo 10.79kg of crude ethanol extract of Bupleurum bupleuri;

[0036] (2) The obtained ethanol crude extract of Bupleurum bupleurum was suspended in water, and sequentially extracted with petroleum ether, ethyl acetate, and n-butanol in equal volume fractions to prepare different polar parts, wherein the n-butanol part was 2.7 kg;

[0037] (3) Mix the n-butanol part obtained in step (2) with 200-300 mesh silica gel according to the n-butanol part and silica gel mass ratio of 1:2, after drying, separate through 200-300 mesh silica gel column chromatography, With chloroform-methanol mixed solvent...

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a novel flavonoid compound extracted and separated from Bupleurum bupleuri and a preparation method thereof. Background technique

[0002] Bupleurum marginatum Wall ex DC. is a plant of the genus Bupleurum in the family Umbelliferae. It has the functions of dispelling wind and reducing fever, soothing the liver and promoting yang. tone, uterine prolapse, anal prolapse, etc. Bamboo leaf Bupleurum is mainly distributed in Sichuan, Hubei, Yunnan, Guizhou and other provinces of China, and it is a local customary product of Bupleurum medicinal materials in southwest my country.

[0003] There are various types of chemical components in Bupleurum bupleuri, mainly containing saponins, flavonoids, lignans, coumarins, and volatile oils. Flavonoids are an important indicator of its quality control. The flavonoids involved in the present invention have no related patents or ...

Examples

Embodiment 1

[0027] Example 1 Preparation of 3,3',5'-trimethoxy-myricetin-7-O-β-D-glucoside from Bupleurum bupleuri

[0028] (1) 15 kg of Bupleurum bupleurum, adding 10 times of volume fraction of 75% ethanol and extracting under reflux for 3 times, each time for 1.5 hours, filtering, merging the extracts, concentrating and recovering the extracts under reduced pressure at 50°C, to obtain bamboo 0.92kg of ethanol crude extract of Bupleurum chinensis;

[0029] (2) Take the ethanol crude extract of Bupleurum bupleuri obtained in step (1) and suspend it in water, and use sherwood oil, ethyl acetate, n-butanol and other volume fractional extractions successively to prepare different polar parts, wherein n-butanol Alcohol part 250g;

[0030] (3) Mix the n-butanol part obtained in step (2) with 200-300 mesh silica gel according to the n-butanol part and silica gel mass ratio of 1:2, after drying, separate through 200-300 mesh silica gel column chromatography, With dichloromethane-methanol mixe...

Embodiment 2

[0034] Example 2 Preparation of 3,3',5'-trimethoxy-myricetin-7-O-β-D-glucoside from Bupleurum bupleuri

[0035] (1) 200 kg of Bamboo Leaf Bupleurum Radix, adding 10 times of volume fraction of 75% ethanol and extracting under reflux for 2 times, each time for 2 hours, filtering, merging the extracts, concentrating and recovering the extracts under reduced pressure at 55° C. to obtain bamboo 10.79kg of crude ethanol extract of Bupleurum bupleuri;

[0036] (2) The obtained ethanol crude extract of Bupleurum bupleurum was suspended in water, and sequentially extracted with petroleum ether, ethyl acetate, and n-butanol in equal volume fractions to prepare different polar parts, wherein the n-butanol part was 2.7 kg;

[0037] (3) Mix the n-butanol part obtained in step (2) with 200-300 mesh silica gel according to the n-butanol part and silica gel mass ratio of 1:2, after drying, separate through 200-300 mesh silica gel column chromatography, With chloroform-methanol mixed solvent...

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