The invention provides an establishment method and application of a red deer pilose
antler cartilage primary cell senescence model, and belongs to the technical field of
biology. According to the invention, a
hydrogen peroxide (H2O2) induction mode is used, and a novel
cell aging model of red deer
antler cartilage primary cells is constructed. And determining that the aging half effect concentration of H2O2 as an
inducer of the cells is 400 mu M, and the
induction time is 2 hours. The
cell senescence model provided by the invention is simple to operate, low in cost, short in
cell induction time, stable in result and high in
repeatability, and experimenters can quickly and effectively construct the cell
senescence model. Importantly, the
cell aging model in the regeneration background can simulate the
oxidative stress state in the aging process of a specific regeneration organ pilose
antler, can be used for screening and research and development of small molecular compounds for preventing and delaying aging, and can deeply compare the mechanisms and functions of regeneration and aging at the
cellular level; and an important cell platform is provided for better understanding the relationship between regeneration and aging.