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Clone seedling breeding method for sea-tangle gametocyte

A gametophyte and kelp technology, applied in botany equipment and methods, seaweed cultivation, plant cells, etc., can solve problems such as long seedling time, seedling time is affected by sea water temperature, mixed kelp varieties, etc., and achieve the effect of increasing production

Inactive Publication Date: 2008-05-14
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Problems in this method: one is that the seedling time is affected by the water temperature in the sea area, and the seedling time is long; the other is that the kelp species are mixed when the kelp is raised; the third is that the development degree and decay of the kelp are different, resulting in the occurrence of different diseases in the seedling process.
[0003] Laminaria gametophyte cloning seedling raising method solves the above-mentioned problems, seedling raising technology has basically matured, but this new technology is difficult to popularize
The reason that causes this situation: the one, because this method requires higher kelp seedling raising technology, the 2nd, can only use single layer curtain in this method seedling raising process, and conventional method can have the double layer curtain of 30~40%, cause cloning Seedling production is reduced by more than 30% compared with conventional methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] Embodiment, a method for cloning seedlings of kelp gametocytes, taking the Bohai Bay area of ​​Jiaodong as an example, on August 9, the circulating water was started to cool down, and the washed and pretreated kelp seedling curtains were neatly placed in the nursery pool On the second day, 30kg of the male and female gametophyte clones stimulated in advance were smashed with a high-speed tissue grinder, and then hand-kneaded into small cell segments with a 400-mesh sieve, and evenly sprayed onto 4000 kelp seedling curtains with a sprayer. Before spraying the clones, fill the nursery pool with water and stop the circulation. On the morning of August 12, the clones were basically attached, and the water began to circulate to provide suitable light, water temperature, water quality, and nutrients to promote the development of the clones; on August 15, the gametocytes began to develop Sporophytes, sporophytes developed to 4 rows of cells on August 25, and some sporophytes we...

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PUM

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Abstract

This invention discloses a clonic seeding method of sea-tangle gametocyte. Comparing with former mature sea-tangle seed source, using gametocyte clone as seed source can solve these problems: seeding time is limited by sea areas and water temperature; seeding time is long; sea-tangle seeding causes its varieties complex; the growth degree and dote condition of sea-tangle are different, which causes various diseas occur during seeding period. The seeding-pick are proceed at twice and two curtains are employed, these heightens sea-tangle seeding output.

Description

1. Technical field [0001] The invention belongs to the technical field of marine organism seedling cultivation, and in particular relates to a method for cloning and seedling cultivation of kelp gametophytes. 2. Background technology [0002] Kelp seedlings still use the conventional method of planting kelp seedlings. This method is to bring the pre-selected mature kelp indoors before the water temperature in the natural sea area rises to 23°C (mid-July in Zhifu Bay). Seawater stimulates the release of zoospores, and after reaching a certain density, the attachment substrate is added. After swimming for a certain period of time, the zoospores actively attach to the substrate. Under conditions such as appropriate water temperature and light, they can begin to grow and develop. After three months The cultivation of kelp seedlings grows to 2 cm, and the natural water temperature in the sea area is stable below 19°C (in mid-October in Zhifu Bay). Move the kelp seedlings out of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01G33/00
Inventor 车轼丛义周于松敏曲善村孙娟金瑜王青岩
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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