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Germ plasm preserving method for kelp

A preservation method and technology of Scherichata, applied in the direction of plant preservation, horticultural methods, botanical equipment and methods, etc., can solve problems such as marine pollution, the population of Scherichia fructus is on the verge of exhaustion, and affects the survival of Scherichia spp. Quantity effect

Inactive Publication Date: 2010-04-14
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Zeng Chengkui and Zhang Junfu analyzed the flora of seaweed (including swan palm) in the 1960s; at present, the natural resources of swan palm are on the verge of exhaustion. We have carried out more than 20 years of investigation and research, especially after recent Years of dragnet searching, only a few Scherichia spp. were found in 2006. It can be seen that the number of this species in the natural sea area is very small, and it is on the verge of exhaustion. The pollution of the gooseberry is becoming more and more serious, and the gooseberry has its reproductive cycle and the requirements of the environmental conditions for reproduction. If the environment is polluted during the reproduction cycle of the gooseberry, it will cause the gooseberry to stop breeding, and the goosefoot will stop breeding. The living environment is polluted, and it will also affect the survival of the swan, causing the population of the swan to be on the verge of exhaustion along the coast of China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A germplasm culture and preservation method of Scherichia cheiferae, comprising the following steps:

[0020] a, culture material: clean and disinfect the leaf of Scherichia chinensis, divide the leaf of Scherichia safflower into strips, weigh each strip as 15g;

[0021] b. Soaking in seawater culture solution: put each 15g leaf of Scherichia chinensis into a glass bottle, add 1000ml seawater culture solution in the glass bottle to soak the leaves of Scherichia chinensis, the seawater culture solution contains 0.5mg / m 3 concentration of CaCl 2 and 5mg / m 3 Concentration of EDTA, N concentration of seawater culture solution is 50mg / m 3 , the ratio of N:P is 16:1, and the salinity is 30‰;

[0022] c. Cultivation and preservation in the incubator: put the glass bottle into the incubator for cultivation and preservation; the temperature in the incubator is controlled within the range of 1-5°C, and the light intensity is 5-10 μE m -2 S -1 , the light time is 15 hours a d...

Embodiment 2

[0025] A germplasm culture and preservation method of Scherichia cheiferae, comprising the following steps:

[0026] a, culture material: disinfect and clean the rhizoids of the gooseberry, divide the rhizomes of the gooseberry into chunks, and weigh each piece as 15g;

[0027] B, soaking in seawater culture solution: put the rhizomes of 15g Scherichia chinensis into a glass bottle, add 300ml seawater culture solution to soak the rhizomes of Scherichia swani in the glass bottle, the seawater culture solution contains 1.0mg / m 3 concentration of CaCl 2 and 12mg / m 3 Concentration of EDTA, N concentration of seawater culture solution is 30mg / m 3 , the ratio of N:P is 13:1, and the salinity is 28‰;

[0028] c. Cultivation and preservation in the incubator: put the glass bottle into the incubator for cultivation and preservation; the temperature in the incubator is controlled within the range of 5-9°C, and the light intensity is 15-25μE m -2 S -1 , the light time is 10 hours ...

Embodiment 3

[0031] A germplasm culture and preservation method of Scherichia cheiferae, comprising the following steps:

[0032] a, culture material: disinfect and clean the stem of Scherichia spp., divide the stem of Scherichia swan into sections, weigh each section as 10g, and then wrap the stem of Scherichia safflower in each section with 8 layers of sterile gauze;

[0033] b. Soaking in seawater culture solution: put the stems of 10g of Scherichia persica into a plastic bag, add 600ml of seawater culture solution in the plastic bag to soak the stems of Scherichia safflower, and the seawater culture solution contains 1.5mg / m 3 concentration of CaCl 2 and 18mg / m 3 Concentration of EDTA, N concentration of seawater culture solution is 80mg / m 3 , the ratio of N:P is 10:1, the salinity is 34‰, soak for 10 minutes, and then remove the seawater culture solution;

[0034] c. Cultivation and preservation in the incubator: put plastic bags into the incubator to cultivate and preserve the ste...

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PUM

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Abstract

The sea-tangle preserving method for kelp includes soaking the leaf, rhizoid or stem of kelp in sea water culture liquid and culturing and preserving in a incubator, replacing sea water culture liquidregularly, and keeping low temperature and long sunshine of low strength to maintain the basic metabolism activity for slow growth and well preservation of the sea-tangle. The present invention favorsthe propagation of kelp and the restoring of biological resource of kelp.

Description

technical field [0001] The invention relates to germplasm preservation of algae, in particular to a germplasm preservation method of Scherichia cheiferae. Background technique [0002] Ecklonia kurome Okam, also known as kelp, black kelp, noodles, black cabbage, hematemesis, etc., belongs to Phaeophyta, unequal generation type, membranous body subclass, kelp order, wing algae, kelp The genus is a warm-water economic seaweed endemic to the western Pacific Ocean. [0003] In history, many scholars have researched Scherichata. In Li Shizhen's "Compendium of Materia Medica" (reprinted according to the Commercial Press in 1954, all six volumes), there are water plants, liverworts and water vegetables. and other categories, in this book, kombu, i.e. gooseberry, was mentioned many times, and it emphasized many advantages of gooseberry as a traditional Chinese medicine, and it also left us many characteristic and symbolic characteristics of gooseberry as a traditional Chinese medic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00A01N3/00C12N5/04A01H4/00
Inventor 骆其君冯婧孙雪龚小敏郑岳夫严小军裴鲁青
Owner NINGBO UNIV
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