Compositions and methods for reducing vaginal ph
A composition and water-based composition technology, applied in the direction of drug combination, active ingredients of artificially synthesized polymer materials, active ingredients of anhydride/acid/halide, etc., can solve the problems of not providing anhydrous composition, low buffer capacity, etc.
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Embodiment 7
[0107] Example 7: Buffer capacity
[0108] To determine the buffer capacity of the compositions of the present invention, the following procedure was used.
[0109] The amount of 0.1 N sodium hydroxide required to change the pH of the compounds described in Examples 1-5 was determined by titration. On a molar equivalent basis, the amount of sodium hydroxide solution added to the sample is indicated in the figure below.
Embodiment 6
[0110] Samples prepared in Example 6 had no buffer capacity between 3.0 and 5.5 and were designed to be delivered with a placebo buffer gel (Example 5) with the dispenser. This is an example of such a two-phase delivery system. As shown, with buffered Metrogel-Vaginal (purchased from 3M Corporation, Minneapolis, Minnesota) to compare the data obtained in the treatment of bacterial vaginosis.
Embodiment 8
[0111] Example 8: In vitro evaluation of biological activity against bacterial vaginosis
[0112] The ability of selected vaginitis anaerobes to survive in a mixture of the agent and supplemented brucella broth was also studied. Two strengths of Brooke's broth supplemented with vitamin K and hemin were prepared and diluted with the formulation of the invention. Study organisms were taken from the refrigerator and subcultured at least twice to ensure their purity and good growth. In vitro evaluations were performed using the following procedure:
[0113] Method: Steer replicator (replicator) test (survival time expressed in hours)
[0114] 1. Mix 1 gram of test sample with 9 milliliters of dimethylsulfoxide ("DMSO"). A preparation was melted at 40-46°C and mixed well before dissolving in DMSO. Prepare 18 ml. Take out a small volume to measure and record the pH. Place in a reaction chamber and exclude from oxygen for at least 2 hours.
[0115] 2. Operate in a reaction cha...
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