Deletion mutant of a set of hepatitis B HBx gene and application thereof

A technology for deletion of mutants and genes, applied in applications, gene therapy, genetic engineering, etc., can solve the problems of clinical diagnosis and treatment without HBx gene deletion mutants

Inactive Publication Date: 2007-10-24
朱平安
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no reports of HBx gene deletion mutations directly causing HCC,...

Method used

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Examples

Experimental program
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Embodiment 1

[0013] Example 1: Deletion Mutant Sequence Determination and Application Research

[0014] 1. Extraction of total DNA from liver cancer tissue

[0015] 63 liver cancer tissue samples were obtained from outpatients and inpatients of General Surgery Department and Department of Infectious Diseases of Xiangya Hospital of Central South University. The patients were from Hunan, Hubei, Jiangxi and other provinces. The patients were positive for HBsAg, but negative for anti-HBs, anti-HCV, and anti-HDV triple antibodies . After surgical operation of these liver cancer tissues, the DNA extraction kit of Beijing Tiangen Biotechnology Co., Ltd. was used to extract the DNA of the liver cancer tissues.

[0016] 2. Nested PCR, single-strand conformation polymorphism analysis (SSCP) and heteroduplex analysis (HA)

[0017] 1) Nested PCR: Nested PCR was used to amplify the HBx gene fragment in the liver cancer tissue, and the primer sequences were:

[0018] P1: 5'-ATCGTTTCCATGGCTGCTAGGCT-3'...

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PUM

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Abstract

The invention provides a deletion mutant of hepatitis B HBX gene, wherein SEQ ID NO. 1 is a mutant lack of 264 bases in 1577-1840 HBV nucleic acid position; SEQ ID NO. 2 is a HBX gene mutant lack of 19 bases in 382-400 nucleic acid position; SEQ ID NO. 3 is a HBX gene mutant lack of 15 bases in 431-445 nucleic acid position. The deletion mutant is provided with apparent oncogenic effect, which can be used to the gene detection of hepatitis B etiological agent carrier and hepatic carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a group of deletion mutants of hepatitis B HBx gene and its application in clinical diagnosis, treatment and prevention. Background technique [0002] Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors in Southeast Asia and China. A large number of epidemiological surveys have shown that long-term chronic hepatitis B virus infection is the main cause of HCC. The risk of HCC in people infected with HBV It is more than 100 times that of uninfected patients, and HCC patients have a tendency to cluster HBV infection in their families, suggesting that HBV infection is closely related to the occurrence of HCC. [0003] HBV is a partially double-stranded DNA virus with a genome of 3.2kb, including S, C, P, and X four open reading frames, among which the X gene is the smallest open reading frame and the region with the most obvious functional overlap. ...

Claims

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Application Information

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IPC IPC(8): C12N15/51C12Q1/68A61K48/00A61P1/16A61P35/00
Inventor 朱平安谭德明陈莉彭忠田宋琳刘菲
Owner 朱平安
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