Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier

A gold nanoparticle and arginine-rich technology is applied in the field of arginine-rich polypeptide-gold nanoparticle cell transport carrier synthesis, which can solve the problems of complex synthesis process, difficulty in combining multiple molecules, long reaction time, etc. Simple, mild conditions and high transfer efficiency

Inactive Publication Date: 2008-01-30
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Application Information

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Problems solved by technology

However, the existing methods of synthesizing gold nanocarriers using peptide chains as the delivery medium often require the use of polymers or proteins to stabilize gold nanoparticles, combined with delivery peptide chains, the synthesis process is often very complicated and requires a long reaction time , and it is difficult to combine multiple molecules to achieve multifunctionality

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  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier
  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier
  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier

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Embodiment 1

[0022] Provided is a method for synthesizing arginine-rich polypeptide polypeptide-gold nanoparticle cell transport carrier. First, according to the Frens-Turkevich method, using sodium citrate to reduce chloroauric acid to prepare gold nanoparticles with a particle size of 30nm; the amino acid sequence is CALNN and CALNNGGRRRRRRR(CALNNR 8 ) polypeptides were mixed, so that the molar concentration ratio of the two polypeptides was 90%: 10%, to obtain the polypeptide mixture; the polypeptide mixture was added to a particle diameter of 30nm gold nano solution, so that the total molar concentration of the polypeptide was 1mM, at room temperature Leave to react for 1 hour, adjust the pH value of the solution to 3 to keep the gold nanoparticles monodispersed in the solution, discard the supernatant after centrifugation at 10,000 rpm, and then add deionized water with a pH value of 3 equal to the supernatant , and finally synthesized the arginine-rich polypeptide-gold nanoparticle c...

Embodiment 2

[0025] Make the peptides CALNN and CALNNGGRRRRRRR(CALNNR 8 ) molar concentration ratio is 0%:100%. Other steps are with embodiment 1

Embodiment 3

[0027] Let the polypeptide and the gold nanometer solution stand at room temperature for 1 hour, adjust the pH value of the solution to 6, keep the gold nanoparticle in the solution monodisperse, discard the supernatant after centrifugation at 10,000 rpm, and then add the same amount of the supernatant deionized water with a pH of 6. Other steps are with embodiment 1

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Abstract

The invention relates to a synthesis method of an arginine-rich polypeptide-gold nanometer carrier. The invention uses a polypeptide surface decorative method and takes a polypeptide to stably cover gold nanometer grain and another type of polypeptide to realize cell cross-membrane transmission function of nanometer grain. The polypeptide-gold nanometer carrier with different transmission capacities can be obtained through regulating proportion of stable polypeptide and transmission polypeptide in primary mixture. The synthesis method has simple operation, short operation time and straightforward detecting method and the synthetic gold nanometer grain carrier has high efficiency of cell cross-membrane transmission.

Description

technical field [0001] The invention relates to a method for synthesizing an arginine-rich polypeptide-gold nano particle cell transport carrier. technical background [0002] Due to its ultra-small size and specific properties (such as fluorescence, magnetism, and compactness), inorganic nanobiotransport systems provide new media and technical means for the acquisition of biochemical processes in living cells and medical targeted drug therapy (Nature Biotechnology, Nat. Biotechnol. 2001, 19, 1013-1017). Among them, the hybrid particles of biomolecules and gold nanoparticles can be used for real-time and dynamic observation of cells due to their biocompatibility and unique optical properties. Therefore, some studies have combined DNA, proteins, antibodies, polymer molecules and gold particles. Transmembrane transport (Science, Science, 2006, 312, 1027-1030; Biopolymer Chemistry, Bioconjugate Chen. 2004, 15, 482-490). In recent years, polypeptide molecules have also been ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63
Inventor 孙琳琳王振新
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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