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Method for extracting toxin from jellyfish tentacle

The technology of jellyfish toxin and tentacles is applied in the field of marine organisms, which can solve the problems of in-depth research, development and utilization of jellyfish toxin, low content of effective components of jellyfish toxin and low biological activity, etc., and achieves high toxicity, strong activity and high concentration. Effect

Active Publication Date: 2008-02-27
水母娘娘海洋生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Existing jellyfish toxin extraction method is to simply extract with tentacle homogenate method, there are many unfavorable factors, at first because more than 90% in jellyfish body is water, contained water has very strong diluting effect to extract; Pollution of the toxin by other tissue and cell components outside the silk vesicles leads to low content of effective components and low biological activity of the extracted jellyfish toxin, which directly affects the in-depth research, development and utilization of jellyfish toxin

Method used

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Examples

Experimental program
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Embodiment

[0020] 1) The jellyfish tentacles were excised from the living jellyfish, and stored at -20°C until use.

[0021] 2) Weigh the frozen jellyfish tentacles of 1000-4000g above and put them in a large beaker, add sterile seawater 2 to 6 times the volume of the jellyfish tentacles (filter the seawater with a 0.22 micron filter membrane, and then boil it to sterilize), 0 Soak at ℃ and stir to dissolve the ice. After standing for 24 hours, pour out the supernatant to wash once. Repeat the above washing for 2 to 6 times to remove the undissolved tissue in the upper layer and collect the sediment in the lower layer for later use.

[0022] 3) Centrifuge the precipitate obtained above at 2-6°C at 3000×g-4800×g for 20-30 min, (see Table 1, speed 1) put the lower sediment into a centrifuge tube, add sterile seawater, and the sediment and The mass volume ratio of seawater is 1:20g / ml, then centrifuge at 10000×g~13000×g for 2~5 times at 2~6℃, each time for 20~30min, observe the precipitate ...

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PUM

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Abstract

The invention discloses a method to extract high activity toxin from jellyfish touching hand in marine biological technique domain, which comprises the following steps: cutting jellyfish touching hand from active jellyfish; adding cooled jellyfish touching hand sea water with 2-6 double volume; self-dissolving; stewing; getting deposition; centrifuging for 20-30 min at 2-6 deg. c with 3000Xg-4800Xg; collecting the deposition; adding into asepsis sea water; centrifuging for 2-5 times once time; collecting the deposition; washing for 3-4 times with NaCl solution; placing into mortar; and adding into Tris-HCl buffer liquid; grinding at room temperature; centrifuging; getting supernatant fluid; getting the jellyfish toxin. This invention can extract a great of barded wire capsule, which can use jellyfish touching hand source completely.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for extracting highly active toxins from collected jellyfish tentacles. Background technique [0002] Jellyfish (Rhopilema esculentum Kishinouye), commonly known as sea snake, belongs to Coelentera, Scyphozoa, Rhizostomeae, and Rhizostmatodae. There are a large number of nematocysts distributed, and the nematocysts contain a large amount of venom, so being stung by jellyfish can cause various physiological reactions, such as itching, local tissue inflammation, shock, and even death. At present, the research on jellyfish mainly focuses on ecology and breeding, while the research on jellyfish toxin is less. Preliminary studies on jellyfish toxins show that jellyfish toxins have various biological activities, such as: hemolytic activity, insecticidal activity, antioxidant activity and enzyme activity. Because of its unique chemical structure and toxicological pro...

Claims

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Application Information

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IPC IPC(8): C07K14/44C07K1/14
Inventor 李鹏程冯金华于华华李翠萍邢荣娥刘松
Owner 水母娘娘海洋生物科技有限公司
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