Detecting method of animals isotope element finger print and uses thereof
A detection method and isotope technology, which is applied in the field of animal isotope fingerprint detection, can solve the problems of information chain interruption, blurred animal labeling patterns, fraud, etc., and achieve the effect of convenient material collection, simple retrospective analysis, and accurate information
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Embodiment 1
[0027] Embodiment 1, establishment of bovine isotope fingerprint detection method
[0028] 1. The effect of isotope fingerprints in the tail hair of each section on the influence of feed ratio during the growth period
[0029] In March 2006, nine 10- to 12-month-old calves were purchased from the cattle trading market in Wukeshu Town Economic Development Zone, Yushu City, Jilin Province, numbered No. 1-9. After purchasing the calf, select a part of the tail of each cow close to the cortex and cut the tail hair as the initial cattle tail hair sample. Then feed in 3 stages according to different feed ratios, and each stage feeds for 3 months (see Table 1 for specific feed ratios). Three of the cows were slaughtered after each stage, and they were slaughtered sequentially from No. 1 to No. 9 (see Table 2 for the specific information and feeding information of each cow). Take the oxtail hair sample from the position where the oxtail hair was clipped in the previous stage, measur...
Embodiment 2
[0053] Embodiment 2, the application effect verification of the method for detecting animal isotopic fingerprints according to the present invention
[0054] 1. Using the method of the present invention to detect animal isotope fingerprints to predict C in cattle feed 4 Proportion of plant feed
[0055] Analysis C 4 The proportion of plant feed and the δ 13 C (see Table 7 for specific values, this data is obtained from the previous cattle model test and the isotope analysis results of cattle tail hair in different regions), the results show that there is a very significant correlation between them, and the correlation coefficient is 0.961 (P13 C value and feed C 4 The relationship equation of the proportion of plants is as follows:
[0056] Y=-12.19X 2 +23.15X-22.79(R 2 =0.992, P<0.01) Formula (1)
[0057] In the formula (1), Y is the δ in the ox tail hair 13 C value; X is C in the feed 4 Proportion of plant feed
[0058] Table 7.C 4 Proportion of plant feed and δ of...
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