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Application of M3 type receptor protein polypeptide resisting antibody measurement in Sjogren syndrome diagnosis

A technology of receptor and polypeptide sequence, which is applied in the field of anti-M3 receptor protein polypeptide antibody detection in the diagnosis of Sjogren's syndrome, and can solve problems such as poor medical compliance, delayed diagnosis, and lost treatment opportunities

Inactive Publication Date: 2008-05-28
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no ideal serological diagnosis method has been found clinically. In addition to clinical symptoms, the diagnosis is mainly made by invasive examinations such as lip gland biopsy and parotid angiography. Due to poor medical compliance of patients, diagnosis is often delayed and the best treatment is lost. opportunity

Method used

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  • Application of M3 type receptor protein polypeptide resisting antibody measurement in Sjogren syndrome diagnosis

Examples

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Effect test

Embodiment 1

[0020] Example 1 Design and Synthesis of Anticholinergic M3 Type Receptor Protein Polypeptide

[0021] The aforementioned research work (1-4) It has been proved that the human cholinergic M3 receptor protein expressed in sf9 cells purchased from sigma can specifically bind to the cholinergic M3 receptor protein antibody in patients with Sjögren's syndrome, and then through specific methods can Check it out. Utilize Flechsler etc. at first in the test of the present invention (5) The reported solid-phase synthesis method synthesized a cholinergic M3-type receptor protein polypeptide with a total of 16 amino acids, and its sequence is as follows: KRTVPPGECFIQFLSE (ie Lys-Arg-Trp-Glu-Val-Pro-Pro-Gly-Phe-Cys -Gln-Phe-Ile-Gln-Phe-Leu-Ser-Glu)

[0022] Studies have shown that the above-mentioned cholinergic M3 receptor protein polypeptide can specifically recognize the specific antibody in the serum of patients with Sjogren's syndrome, which is of great significance for the diagn...

Embodiment 2

[0023] Example 2 Detection of Anticholinergic M3 Receptor Protein Polypeptides to Antibodies in Serum of Patients with Sjögren's Syndrome

[0024] In this study, enzyme-linked immunosorbent assay (ELISA) was used to detect anti-M3 receptor protein polypeptide antibodies in the serum of patients with Sjögren's syndrome, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) to evaluate The distribution of the polypeptide antibody in various diseases.

[0025] First, the M3 receptor protein polypeptide is coated on an ELISA microwell plate and incubated with the serum to be tested. If there is an anti-M3 receptor protein polypeptide antibody in the serum, the antibody will bind to the coated solid. Combine with antigen to form antigen-antibody complex. After washing the unbound antibody and other components, add horseradish peroxidase (HRP)-labeled goat anti-human IgG antibody (IgG-HRP) and incubate together. At this time, IgG-HRP will bind to the antigen-antibody co...

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Abstract

The invention provides a check of Sjogren syndrome by M3-type receptor protein polypeptide and analogues, wherein the peptide antigen can be specially combined with specific antibody in Sjogren syndrome patient, with high sensitivity and specificity.

Description

technical field [0001] The invention relates to the preparation of cholinergic M3 type receptor polypeptide and its application as an antigen in detecting Sjögren's syndrome and other diseases. 1. Background Technology [0002] Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by invading exocrine glands such as lacrimal glands and salivary glands. The most common clinical manifestations of the disease are dry eyes and dry mouth, and can involve the lungs, kidneys, lymphatic system, blood vessels and liver and other organs. The disease has no obvious regional distribution difference, and can occur at any age, with 40-60 years old, especially postmenopausal women in the majority, and the ratio of male to female is 1:9. The prevalence rate in my country is 0.77%, and the prevalence rate in the elderly population is as high as 4%. There are nearly 8 million patients in the whole country. Sjogren's syndrome can be divided into two types: primary Sjogren's ...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/566
Inventor 栗占国
Owner PEOPLES HOSPITAL PEKING UNIV
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