Biological sample glass freezing and conserving appliance

A technology for vitrifying and freezing biological samples, which is applied in the field of tools to reduce the loss of liquid nitrogen, make up for the difficulty of drawing samples, and store a large number of samples.

Inactive Publication Date: 2008-06-18
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem that the biological samples can be quickly vitrified, and the biological samples are not directly exposed to liquid nitrogen to prevent potential biological contamination, the present invention provides a vitrification and preservation method for biological samples such as eggs and embryos. tool

Method used

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  • Biological sample glass freezing and conserving appliance
  • Biological sample glass freezing and conserving appliance

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The biological sample vitrification and preservation tool includes a straw 1 and a sheath tube 2, and the sheath tube 2 is set in the middle of the straw 1, as shown in Figures 1 and 2;

[0026] The length of the straw is 90mm, and the diameter at both ends is Φ2.0mm. The middle part is a thin and thin-walled tube. The length of the thin and thin-walled tube in the middle is 30mm, and the diameter is Φ0.35mm. The material of the straw is polypropylene.

[0027] The length of the sheath tube is 50mm, and the diameter is Φ2.55mm; both sides of the middle part are symmetrically provided with concave windows with a length of 35mm and a width of 1.5mm, as shown in Figure 3, Figure 5 and Figure 6. The material of the sheath tube is metal material.

[0028] The preferred vitrification and thawing procedures for eggs or embryos and cells are as follows. Eggs or embryos and cells are suspended in the equilibration solution for 5 minutes and then transferred to vitrification so...

Embodiment 2

[0031] The biological sample vitrification freezing and preservation tool includes a straw 1 and a sheath tube 2, and the sheath tube 2 is set in the middle of the straw 1;

[0032] The straw 1 has a length of 80 mm and a diameter of Φ1.5 mm at both ends. The middle part is a slender thin-walled tube 3 with a length of 20 mm and a diameter of Φ0.3 mm; the slender thin-walled tube part is used to carry biological samples the bearing part;

[0033] The sheath tube 2 has a length of 45 mm and a diameter of Φ2.05 mm; the two sides of the middle part are symmetrically provided with concave windows 4 with a length of 30 mm and a width of 1.0 mm.

[0034] Others are with embodiment 1.

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Abstract

The present invention relates to a tool for vitrification freezing and storing biological samples of ovum, embryo, etc. The present invention solves the problem that the biological sample is frozen promptly but is not exposed to liquid nitrogen directly, so as to prevent the potential biological pollution danger. The tool for vitrification freezing and storing biological sample comprises a straw and a protecting pipe which is mounted at the straw. The middle part of the straw is a fine thin-wall tube, the length of which is 20 to 30mm, and the diameter is 0.3 to 0.35mm. The two sides at the middle part of the protecting pipe are symmetrically provided with concave windows. The straw tool of the present invention is used for vitrification freezing and storing the ovum or the embryo and other cells, which can ensure that the biological sample is vitrified and frozen fast and also can ensure that the biological sample is not exposed to the liquid nitrogen directly to prevent the potential biological pollution danger.

Description

technical field [0001] The present invention relates to tools used in vitrification and preservation of ova and embryos and other cells. Background technique [0002] Freezing of human eggs and embryos is a powerful therapeutic and fertility preservation method in assisted reproductive technology. In particular, cryopreservation of eggs is a viable method of fertility preservation for women at risk of ovarian function loss due to side effects of pelvic disease, radiation, and chemotherapy. Although there have been reports of pregnancy and childbirth using human frozen eggs, the cryopreservation technology of human eggs is still in the development stage. The current vitrification technology can improve the survival rate of human oocytes after thawing and obtain effective pregnancy and live birth. [0003] Cell vitrification technology, that is, in the process of cooling, the water in the living cells does not form ice crystals and directly becomes a glass-like solidificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08A01N1/02
Inventor 曹云霞千日成
Owner ANHUI MEDICAL UNIV
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