Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological sample glass freezing and conserving appliance

A technology for vitrifying and freezing biological samples, which is applied in the field of tools to reduce the loss of liquid nitrogen, make up for the difficulty of drawing samples, and store a large number of samples.

Inactive Publication Date: 2010-09-29
ANHUI MEDICAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the biological samples can be quickly vitrified, and the biological samples are not directly exposed to liquid nitrogen to prevent potential biological contamination, the present invention provides a vitrification and preservation method for biological samples such as eggs and embryos. tool

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological sample glass freezing and conserving appliance
  • Biological sample glass freezing and conserving appliance
  • Biological sample glass freezing and conserving appliance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The tool for vitrification and preservation of biological samples includes a straw 1 and a sheath tube 2, and the sheath tube 2 is set in the middle of the straw 1, see figure 1 with figure 2 ;

[0026] The length of the straw is 90mm, and the diameter at both ends is Φ2.0mm. The middle part is a slender thin-walled tube. Figure 4 . The material of the straw is polypropylene.

[0027] The length of the sheath tube is 50mm, and the diameter is Φ2.55mm; both sides of the middle part are symmetrically provided with concave windows with a length of 35mm and a width of 1.5mm, see image 3 , Figure 5 with Image 6 . The material of the sheath tube is metal material.

[0028] The preferred vitrification and thawing procedures for eggs or embryos and cells are as follows. Eggs or embryos and cells are suspended in the equilibration solution for 5 minutes and then transferred to vitrification solution. Within 45-60 seconds, the ovum or embryo, cells and vitrified liq...

Embodiment 2

[0031] The biological sample vitrification freezing and preservation tool includes a straw 1 and a sheath tube 2, and the sheath tube 2 is set in the middle of the straw 1;

[0032] The straw 1 has a length of 80 mm and a diameter of Φ1.5 mm at both ends. The middle part is a slender thin-walled tube 3 with a length of 20 mm and a diameter of Φ0.3 mm; the slender thin-walled tube part is used to carry biological samples the bearing part;

[0033] The sheath tube 2 has a length of 45 mm and a diameter of Φ2.05 mm; the two sides of the middle part are symmetrically provided with concave windows 4 with a length of 30 mm and a width of 1.0 mm.

[0034] Others are with embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
lengthaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to a tool for vitrification freezing and storing biological samples of ovum, embryo, etc. The present invention solves the problem that the biological sample is frozen promptly but is not exposed to liquid nitrogen directly, so as to prevent the potential biological pollution danger. The tool for vitrification freezing and storing biological sample comprises a straw and a protecting pipe which is mounted at the straw. The middle part of the straw is a fine thin-wall tube, the length of which is 20 to 30mm, and the diameter is 0.3 to 0.35mm. The two sides at the middle part of the protecting pipe are symmetrically provided with concave windows. The straw tool of the present invention is used for vitrification freezing and storing the ovum or the embryo and other cells, which can ensure that the biological sample is vitrified and frozen fast and also can ensure that the biological sample is not exposed to the liquid nitrogen directly to prevent the potential biological pollution danger.

Description

technical field [0001] The present invention relates to tools used in vitrification and preservation of ova and embryos and other cells. Background technique [0002] Freezing of human eggs and embryos is a powerful therapeutic and fertility preservation method in assisted reproductive technology. In particular, cryopreservation of eggs is a viable method of fertility preservation for women at risk of ovarian function loss due to side effects of pelvic disease, radiation, and chemotherapy. Although there have been reports of pregnancy and childbirth using human frozen eggs, the cryopreservation technology of human eggs is still in the development stage. The current vitrification technology can improve the survival rate of human oocytes after thawing and obtain effective pregnancy and live birth. [0003] Cell vitrification technology, that is, in the process of cooling, the water in the living cells does not form ice crystals and directly becomes a glass-like solidificatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/06C12N5/08A01N1/02
Inventor 曹云霞千日成
Owner ANHUI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com