Method for preparing 1-aminohydantoin artificial antigen

A technology of aminohydantoin and artificial antigen, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of only instrument detection, retention, and detection technology that cannot meet the requirements, and achieve The effect of simple synthesis steps

Inactive Publication Date: 2008-07-09
JIANGNAN UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Benefits of technology

This patented technology allows scientists to create an easy-to use tool that helps researchers learn about their own body chemistry better than what they already know from other sources like blood or urine samples.

Problems solved by technology

The technical problem addressed by this patented research is how to develop better ways to detect small amounts of substances called aminohydroacidones that may cause harm or damage when applied to humans during medical procedures such as chemotherapy treatment. Current methods require expensive equipment like gas chromatography analysis, which makes them difficult to implement at home without specialized training staffing. Therefore, developing new techniques for identifying these chemical agents has become important issues.

Method used

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  • Method for preparing 1-aminohydantoin artificial antigen
  • Method for preparing 1-aminohydantoin artificial antigen
  • Method for preparing 1-aminohydantoin artificial antigen

Examples

Experimental program
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Embodiment 1

[0031] (1) Preparation of artificial hapten:

[0032] Weigh 3g (0.026mol) of 1-aminohydantoin, add distilled water 10mL, 1mol / L HCl5mL and p-aldehyde benzoic acid 2g (0.013mol) in a beaker equipped with a magnetic stirrer device, slowly add N , N-dimethylformamide (DMF) until the p-aldehyde benzoic acid is completely dissolved, add 1-aminohydantoin 3g (0.026mol) while stirring, the entire reaction temperature is controlled at 20-30°C, and the reaction is controlled for 2 hours .

[0033] Purification: filter the reaction solution to obtain a white solid, wash with 10mL distilled water and then filter, repeat 2-3 times, put it in a vacuum drying oven and dry at 30°C to obtain the artificial hapten 1-aminohydantoin paraformyl benzoic acid (CPAHD).

[0034] Reaction end point monitoring: for the production of silica gel thin-layer plates, weigh 12g of silica gel, dissolve it in 40ml of 0.5% mass concentration sodium hydroxymethyl cellulose solution, fully stir it into a paste w...

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Abstract

A process for preparing 1-amino hydantoin artificial antigen belongs to the technical field of biochemical industry, which comprises using 1-amino hydantoin artificial antigen as raw material, reacting with p-aldehydobenzoic acid as stirring, synthesizing semi-antigen 1-amino hydantoin-p-aldehydobenzoic acid (CPAHD), coupling carboxyl on semi-antigen and amino on bovine serum albumin (BSA) through adopting carbodiimide method, thereby preparing artificial antigen. The invention synthesizes artificial antigen of 1-amino hydantoin, and the synthetic steps are simple and effective. The invention can be completely applied in immune analysis, provides a convenient method for further research, and can meet domestic research demands.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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