Method for preparing 1-aminohydantoin artificial antigen

A technology of aminohydantoin and artificial antigen, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of limited instrument detection, stay, and the detection technology cannot meet the requirements, etc. The effect of simple synthesis steps

Inactive Publication Date: 2010-11-03
JIANGNAN UNIV
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Problems solved by technology

Due to the illegal use of this prohibited drug, it causes residues in the animal body, but the current domestic detection technology mostly stays in the detection of the original drug, and there are few researches on the detection of metabolites, and some are limited to instrument detection
With the improvement of international standards and the internationalization of my country's trade, this detection technology can no longer meet the requirements. It is necessary to study a more effective and fast detection method-enzyme-linked immunosorbent technology (ELISA)
So far, there is no artificial antigen report for ELISA detection of nitrofurantoin metabolite 1-aminohydantoin in China. In order to fill this gap, it is necessary to provide an effective artificial antigen for 1-aminohydantoin. Antigen preparation method

Method used

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  • Method for preparing 1-aminohydantoin artificial antigen
  • Method for preparing 1-aminohydantoin artificial antigen
  • Method for preparing 1-aminohydantoin artificial antigen

Examples

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Embodiment 1

[0031] (1) Preparation of artificial hapten:

[0032] Weigh 3g (0.026mol) of 1-aminohydantoin, add distilled water 10mL, 1mol / L HCl5mL and p-aldehyde benzoic acid 2g (0.013mol) in a beaker equipped with a magnetic stirrer device, slowly add N , N-dimethylformamide (DMF) until the p-aldehyde benzoic acid is completely dissolved, add 1-aminohydantoin 3g (0.026mol) while stirring, the entire reaction temperature is controlled at 20-30°C, and the reaction is controlled for 2 hours .

[0033] Purification: filter the reaction solution to obtain a white solid, wash with 10mL distilled water and then filter, repeat 2-3 times, put it in a vacuum drying oven and dry at 30°C to obtain the artificial hapten 1-aminohydantoin paraformyl benzoic acid (CPAHD).

[0034] Reaction end point monitoring: for the production of silica gel thin-layer plates, weigh 12g of silica gel, dissolve it in 40ml of 0.5% mass concentration sodium hydroxymethyl cellulose solution, fully stir it into a paste w...

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Abstract

A process for preparing 1-amino hydantoin artificial antigen belongs to the technical field of biochemical industry, which comprises using 1-amino hydantoin artificial antigen as raw material, reacting with p-aldehydobenzoic acid as stirring, synthesizing semi-antigen 1-amino hydantoin-p-aldehydobenzoic acid (CPAHD), coupling carboxyl on semi-antigen and amino on bovine serum albumin (BSA) through adopting carbodiimide method, thereby preparing artificial antigen. The invention synthesizes artificial antigen of 1-amino hydantoin, and the synthetic steps are simple and effective. The inventioncan be completely applied in immune analysis, provides a convenient method for further research, and can meet domestic research demands.

Description

technical field [0001] The invention discloses a preparation method of 1-aminohydantoin artificial antigen, which belongs to the technical field of biochemical industry. Background technique [0002] 1-Aminohydantoin, English name: 1-aminohydantoin hydrochloride (AHD), CAS#2827567, structural formula is NH 2 NCH 2 CONHCO is the metabolite of nitrofuran drug nitrofurantoin in animals. Nitrofuran drugs can be used to prevent and control aquatic animal diseases and disinfection of aquatic animal breeding environment, and can also be used to prevent and treat intestinal infection of livestock and poultry, and are widely used. After the nitrofuran drugs enter the animal body, they decompose quickly due to their poor stability, and combine with animal tissue proteins in the form of metabolites to form stable compounds. Due to the strong toxicity and carcinogenic side effects of nitrofuran drugs, countries all over the world have paid great attention and banned their use. Due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07D233/80C07K1/02
Inventor 胥传来徐一平李秋生袁媛
Owner JIANGNAN UNIV
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