Prediction method of rice hybrid yield heterosis
A prediction method and hybrid technology, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that it is difficult to achieve effective prediction methods and technology breakthroughs in rice heterosis, so as to improve breeding efficiency and reduce errors , the effect of improving predictability
Inactive Publication Date: 2008-07-30
ZHEJIANG SCI-TECH UNIV
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The reason is that the heterosis trait of rice is a non-single trait. It runs through heterosis from single-cell heterozygotes to tissues, organs, and even the entire plant, and the growth period. It is impossible for a specific gene or genes Manipulating het
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Abstract
The invention discloses a forecasting method of the yield advantages of rice hybrids, which includes the following steps: (1) oligonucleotide sequence can be synthesized; (2) connexons can be prepared; (3) the DNA of the gene group of a first generation hybrid can be extracted; (4) the DNA of the parental gene group can be connected with the connexons; (5) PCR amplification reaction can be conducted; (6) the outcomes of the PCR can be conducted the quantitative analysis; (7) the yield advantages of rice hybrid can be forecasted. According to the molecular weight corresponding to the peak value of the outcomes of the PCR of the first generation hybrid, the more the molecular weight is, the stronger the yield advantages of the first generation hybrid is. The forecasting method of the invention can be used for learning the yield advantages of rice hybrids quickly and accurately.
Description
technical field [0001] The invention relates to a crop breeding technology, in particular to a method for predicting rice heterosis yield. Background technique [0002] Crop heterosis is a common phenomenon in nature. It is mainly manifested in the first generation of hybrids, which have obvious advantages compared with their parents. In the past few decades, the use of crop heterosis has significantly improved crop yield and adaptability, especially the successful use of rice heterosis in my country has attracted worldwide attention and has become one of the greatest achievements in the field of agricultural research in the last century. For a long time, breeders at home and abroad have maintained great interest in the discovery and utilization of rice heterosis. However, due to the particularity and complexity of rice heterosis traits, heterosis breeding is more cumbersome and complicated than conventional breeding, especially the evaluation of heterosis yield is time-con...
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IPC IPC(8): C12Q1/68
Inventor 刘小川
Owner ZHEJIANG SCI-TECH UNIV
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