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Rieger Begonia tissue culture quick replication method

A tissue culture and rapid technology, applied in the field of bioengineering, can solve the problems of long induction time, low seedling induction rate, and inability to adapt to market demand, etc., and achieve the effect of convenient induction and cultivation and good effect

Inactive Publication Date: 2008-10-29
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the emergence induction rate of prior art is not high; And induction time is also longer, can not adapt to more rapid market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements. Specifically: take the young leaves of Rieger Begonia with red flowers, wash them with tap water for 20-30 minutes, and then soak them with detergent-added water for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, soak in 70% alcohol for 20 seconds, and then soak in 10% (V / V) sodium hypochlorite (available chlorine ≥ 7.5%) solution with Tween-20 for 10 minutes After the leaves are sterilized, put them into sterile bottles for later use. When necessary, the leaves are taken out from the aseptic bottle, and after removing the edge and petiole of the leaves on the ultra-clean workbench, they are then cut into leaf pieces of 0.5-1 cm×0.5-1 cm in size to form explants.

[0031] 2. Inducing budding: inoculate the leaf pieces as explants on the medium with the formula MS+6-benzylamino...

example 2

[0034] Get the young leaves of Rieger Begonia with pink flowers, and process them in the same way as Example 1 to obtain explants. Then the explants are inoculated on the same medium as in Example 1, placed under the light of 100~200lx and cultivated for 14 days, and then moved to the light of 1100~1200lx and cultivated. After 18 days, the induction rate of clustered buds reaches 99%. After 21 days, the induction rate of clustered buds reached 100%.

example 3

[0036] Get the young leaves of Rieger Begonia with red flowers, and process them in the same way as example one to obtain explants. Then the explants were inoculated on the medium whose formula was MS+6-benzylaminopterin 0.6mg / l+ naphthalene acetic acid 0.3mg / l+ sucrose 30g+ agar 6g, first cultured under 100~200lx light conditions for 14 days, and then transplanted When cultivated under the light condition of 1100~1200lx, the induction rate of clustered buds reaches 90% after 18 days, and the induction rate of clustered buds reaches 100% after 28 days.

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PUM

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Abstract

The invention relates to a rapid propagation method for culturing Rieger begonias tissue, which comprises the following steps that: explant material is treated, that is, the explant material is selected for treatment to prepare the explant meeting the requirement; then inducement is carried out, that is, the explant prepared in the first step gets vaccinated to culture medium to induce bud on inducing conditions; the inducing condition that the light intensity with illuminance is less than 1500lx is met. As the lighting condition during the fast propagation of Rieger begonias tissue is further optimized, the application of the rapid propagation method for culturing Rieger begonias tissue causes the inducement time to be shorter and ensures the inducement rate to be higher and reach 100 percent.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to plant tissue culture technology, in particular to a rapid propagation method of Rieger crabapple tissue culture. Background technique [0002] Rieger begonia (Rieger begonias), also known as rose begonia, is a perennial herbaceous flower of Begoniaceae Begonia. It is a hybrid strain of bulbous begonia and wild begonia, and belongs to high-grade potted flowers. Generally, seeds cannot be formed, and most of them are propagated by cuttings. However, Rieger Begonia has strict temperature requirements, and is afraid of heat and cold. The quality of cutting propagation is not ideal. The technology of tissue culture propagating plants is a special vegetative propagation method, which is now generally called rapid propagation technology or micropropagation technology. It is a method of propagating plants under sterile conditions by using part of the plant body, including cells, ti...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 李晓东卢爱华邓盾
Owner SHENZHEN POLYTECHNIC
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