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Efficient flameray-gerbera propagation production method using excised leaf

A technology of isolated leaves and gerbera, applied in the field of plant biology, can solve the problems that new varieties cannot be quickly popularized and applied, the propagation cycle is long, and the production efficiency is low, so as to improve the multiplication efficiency and production efficiency, shorten the production cycle, accelerate the applied effect

Inactive Publication Date: 2009-02-11
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the practical problems of low multiplication rate, long multiplication cycle, low production efficiency, independent varieties and new varieties introduced from abroad in the plant tissue culture link of the prior art, and provides a high-efficiency and fast Method for breeding and producing gerbera tissue culture seedlings

Method used

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  • Efficient flameray-gerbera propagation production method using excised leaf
  • Efficient flameray-gerbera propagation production method using excised leaf
  • Efficient flameray-gerbera propagation production method using excised leaf

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Selection of explants: From the planted gerbera (the gerbera variety is selected from the variety that has obtained the new variety right of gerbera in China, and its variety right number is CNA20030516.6), it is selected to grow robustly, have stable performance and no pests and diseases Small flower buds of 0.5-1.5 cm are picked from the excellent individual plants, and the flower buds are not exposed or the sepals are not unfolded;

[0024] 2. Establishment of a sterile explant system: Peel off the outer sepals of the above-mentioned gerbera small flower buds and keep a little pedicel, wash in a solution with an appropriate amount of detergent and fully rinse with clear water, then wash in 0.15% mercuric chloride solution Sterilize for 18 minutes, peel off all the sepals, then sterilize in 2.0% sodium hypochlorite solution for 12 minutes, wash with sterile water twice, and set aside for use. When disinfecting, fully shake the bottle so that the sterilized material ...

Embodiment 2

[0032]1. Selection of explants: From the planted gerbera (the gerbera variety is selected from the variety that has obtained the new variety right of gerbera in China, and its variety right number is CNA20030516.6), it is selected to grow robustly, have stable performance and no pests and diseases Small flower buds of 0.5-1.5 cm are picked from the excellent individual plants, and the flower buds are not exposed or the sepals are not unfolded;

[0033] 2. Establishment of a sterile explant system: Peel off the outer sepals of the above-mentioned gerbera small flower buds and keep a little pedicel, wash in a solution with an appropriate amount of detergent and fully rinse with clear water, then wash in 0.15% mercuric chloride solution Sterilize for 20 minutes, peel off all the sepals, then sterilize in 2.0% sodium hypochlorite solution for 10 minutes, wash 3 times with sterile water, and wait for use. When disinfecting, shake the bottle fully to make the disinfectant fully conta...

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Abstract

The invention provides a high-efficiency propagation African daisy-producing method by utilizing in vitro blades, which comprises the selection of an explant, the establishment of an aseptic explant system, the induction of adventitious bud and secondary multiplication culture, particularly comprises the induction culture of the in vitro blades, the secondary multiplication culture of the adventitious bud on the in vitro blades, and the rootage culture of the adventitious bud. The method greatly improves reproduction and production efficiency, shortens seedling production period, and accelerates the application of new species; by statistics, the inductivity and the reproduction rate of the blades after being cultured for 1 month are respectively 85.4 to 85.9 percent and 34.9 times; the method solves the problems of the existing African daisy in the tissue culture rapid propagation production that the propagation speed is low and the seedling quality is poor, etc., the propagation efficiency and the production efficiency of the tissue culture rapid propagation can be improved within short period, the propagation and rootage efficiencies of 50 days are respectively 8.4 to 10.8 times and 129 and 100 strain per block; furthermore, the aseptic blade does not need disinfection, has more material, can be carried out in any stage of the tissue culture rapid propagation, and has actual application value to self-culture and the generalization of new species, the rapid propagation of detoxication original seed, and the rejuvenation of production species.

Description

technical field [0001] The invention relates to a high-efficiency propagation and production method of gerbera, belonging to the field of plant biotechnology. Background technique [0002] Gerbera is one of the top five cut flowers in the world, and it is also the main cut flower in the flower industry in Yunnan Province. In recent years, the production and quality of fresh cut flowers in my country have developed rapidly. In Yunnan Province alone, nearly 10 million seedlings are needed every year. The adventitious bud proliferation technology currently used in tissue culture production is used for propagation, and the proliferation rate is 2.3-5.0 times . In addition, the current domestically produced gerbera varieties are all foreign varieties. Only by introducing them from abroad, not only will you have to pay expensive variety fees, but you will also have a long period of bud induction, low efficiency, low proliferation rate of adventitious buds, and slow propagation spe...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 吴丽芳李绅崇杨春梅蒋亚莲王继华张艺萍吴学尉崔光芬
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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