Method for extracting and preparing total polysaccharide of compound abnormal savda mature agent
A technology of total polysaccharides and ripening agents, which is applied in the field of extraction and preparation of total polysaccharides, can solve the problems of high total polysaccharide content in abnormal black bile maturation agents, achieve low cost, wide application range, and demonstrate the effect of technological progress
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Embodiment 1
[0017] Take 3kg of dry powder of abnormal black gallbladder mature agent, add 15000mL of 90 ℃ hot water, stir for 30 minutes to fully dissolve, filter while hot, concentrate the filtrate under reduced pressure to 6000mL, pass through AB-8 macroporous adsorption resin column, and dissolve it with 30000mL Elute with water, collect the water eluate, add 2% activated carbon for decolorization and purification, filter, concentrate the filtrate under reduced pressure to 6000mL, cool the concentrated solution to 10°C, add 12000mL of 95% ethanol, stir for 10 minutes, and stand at 4°C for 24 hours, a precipitate formed, which was obtained by filtration. Add 5000ml of 90°C hot water to the precipitate, stir for 30 minutes to dissolve, filter while it is hot, concentrate the filtrate under reduced pressure to a relative density of 1.21, cool the concentrate to 10°C, add 2 times the amount of 95% ethanol, stir for 10 minutes, After standing at ℃ for 24 hours, a precipitate was formed, and...
Embodiment 2
[0019] Take 3 kg of dry powder of compound abnormal black gallbladder mature agent, add 18000 mL of 85 ° C hot water, stir for 40 minutes to fully dissolve, filter while hot, and concentrate the filtrate to 6500 mL under reduced pressure, pass through D-101 macroporous adsorption resin column, and Elute with 40000mL of water, collect the eluate, add 2% activated carbon for decolorization and purification, filter, concentrate the filtrate under reduced pressure to 6500mL, cool the concentrated solution to 10°C, add 12500mL of 95% ethanol, stir for 15 minutes, and stand at 5°C After 36 hours, a precipitate formed, which was obtained by filtration. Add 6000ml of 92°C hot water to the precipitate, stir for 20 minutes to dissolve, filter while it is hot, concentrate the filtrate under reduced pressure to a relative density of 1.19, cool the concentrate to 4°C, add 1.5 times the amount of 95% ethanol, stir for 20 minutes, After standing still for 36 hours, a precipitate was formed, ...
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