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Construction method of blood capillary model and microsystem chip thereof

A microchip and chip technology, applied in interdisciplinary fields, can solve problems such as the lack of capillary tissue structure

Inactive Publication Date: 2011-06-08
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current research is only limited to the cell level, and no structure similar to capillary tissue has been established on the basis of endothelial cell culture, and deeper tissue engineering research is carried out

Method used

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  • Construction method of blood capillary model and microsystem chip thereof
  • Construction method of blood capillary model and microsystem chip thereof
  • Construction method of blood capillary model and microsystem chip thereof

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Embodiment Construction

[0017] A specific implementation process of the present invention will be further described below in conjunction with the accompanying drawings.

[0018] In the following examples, the extracellular matrix and intracellular matrix used refer to the solution environment outside and inside the two rows of cells respectively.

[0019] Such as figure 2 As shown, the cell culture microchip provided in this aspect is formed by bonding a polydimethylsiloxane (PDMS) chip and a glass slide, and the PDMS chip includes a PDMS substrate and 2 pairs (4) on it. Micro-dam structure, 2 pairs of micro-dam structures with a spacing of 100-120um, each pair of micro-dam structures includes 2 micro-dams with a spacing of 30-40um, and the gap height between the micro-dam and the glass slide is 3-5um, thus, The space in the microdam structure can be used for fixed culture of cells, such as endothelial cells, to form 2 rows (one row can be formed in each microdam structure) monolayer cell structure...

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Abstract

The invention discloses a method for constructing a blood capillary model and a microsystem chip thereof. The invention utilizes microsystem processing technology to design and manufacture a microchip system for ordered arrangement and culture of endothelial cells of a blood capillary. The method constructs the blood capillary model with normal physiological functions by the following three main steps: design and manufacture of the microsystem chip, transfer, arrangement, fixation and culture of the endothelial cells of the blood capillary, and measurement of the functions of the blood capillary model. The blood capillary model can be used for culturing and observing the endothelial cells of the blood capillary, can perform real-time measurement on the concentration of vasodilative factors NO excreted by the endothelial cells, can make the permeability of the tube wall changed along with different additional stimuli, approximately simulate the functions of the blood capillary under normal physiological conditions, and can be used for sieving active ingredients of cardiovascular drugs.

Description

technical field [0001] The invention relates to a method for constructing a capillary model and a microsystem chip thereof. The model is constructed based on microsystem technology combined with cell biology technology, and can be used in drug screening, tissue engineering, basic cell biology, etc., and belongs to the field of interdisciplinary technology . Background technique [0002] Capillary is the end of the blood circulation. The capillary wall is mainly composed of a layer of endothelial cells (endothelial cells) and basement membrane (Basal Lamina). The thin capillary cross-section is surrounded by an endothelial cell, and the thick capillary Surrounded by 2 to 3 endothelial cells. Conventional capillary models generally use biodegradable materials as scaffolds to cultivate normal cells in the vessel wall, and spin culture reconstruction and regeneration to form tubular models. At present, a variety of tissue-engineered blood vessel construction models are being s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M3/00
Inventor 金庆辉邵建波郑允焕吴建璋赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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